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- KD/KO Validated
GJB3 Polyclonal antibody
GJB3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 12880-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse ovary tissue, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervix tissue, human ovary tumor tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
12880-1-AP targets GJB3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, marmoset, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GJB3 fusion protein Ag3839 |
| Full Name | gap junction protein, beta 3, 31kDa |
| Calculated molecular weight | 270 aa, 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC012918 |
| Gene symbol | GJB3 |
| Gene ID (NCBI) | 2707 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GJB3, also known as Connexin-31 (Cx31), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Mutations in the gene of GJB3 have been described in patients with dominant and recessive hearing impairment and in patients with erythrokeratodermia variabilis (EKV).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GJB3 antibody 12880-1-AP | Download protocol |
| IHC protocol for GJB3 antibody 12880-1-AP | Download protocol |
| IF protocol for GJB3 antibody 12880-1-AP | Download protocol |
| IP protocol for GJB3 antibody 12880-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genes Cells Skp2 contributes to cell cycle progression in trophoblast stem cells and to placental development. | ||
Sci Rep Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea. | ||
Stem Cells FBXL12-Mediated Degradation of ALDH3 Is Essential for Trophoblast Differentiation during Placental Development. | ||
Cell Rep Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss. |
