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GJB3 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 12880-1-AP

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Con: 36 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

GJB3 Antibody WB mouse ovary tissue 12880-1-AP

mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12880-1-AP(GJB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

GJB3 Antibody IP HeLa cells 12880-1-AP

IP result of anti-GJB3(IP:12880-1-AP, 4ug; Detection:12880-1-AP 1:500) with HeLa cells lysate 2600 ug

GJB3 Antibody IHC human cervix tissue 12880-1-AP

Immunohistochemistry of paraffin-embedded human cervix tissue slide using 12880-1-AP( GJB3 Antibody) at dilution of 1:50 (under 10x lens)

GJB3 Antibody IHC human cervix tissue 12880-1-AP

Immunohistochemistry of paraffin-embedded human cervix tissue slide using 12880-1-AP( GJB3 Antibody) at dilution of 1:50 (under 40x lens)

GJB3 Antibody IF HeLa cells 12880-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 12880-1-AP(GJB3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, marmosets
Positive WB detected in:
mouse ovary tissue, HeLa cells, human brain tissue, human heart tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human cervix tissue, human ovary tumor tissue, human skin cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GJB3 fusion protein Ag3839
Full name:
gap junction protein, beta 3, 31kDa
Calculated molecular weight:
270aa,31 kDa
Observed molecular weight:
31 kDa
GenBank accession number:
BC012918
Gene ID (NCBI):
2707
Gene symbol
GJB3
Synonyms
Connexin 31, CX31, DFNA2, DFNA2B, EKV, Gap junction beta 3 protein, GJB3

Background

GJB3, also known as Connexin-31 (Cx31), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Mutations in the gene of GJB3 have been described in patients with dominant and recessive hearing impairment and in patients with erythrokeratodermia variabilis (EKV).


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