GJB3 Polyclonal antibody

GJB3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12880-1-AP

Synonyms

Connexin 31, CX31, DFNA2, DFNA2B, EKV, Gap junction beta 3 protein, GJB3



Tested Applications

Positive WB detected inmouse ovary tissue, HeLa cells, human brain tissue, human heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervix tissue, human ovary tumor tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12880-1-AP targets GJB3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, marmosets, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GJB3 fusion protein Ag3839
Full Name gap junction protein, beta 3, 31kDa
Calculated molecular weight 270 aa, 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC012918
Gene symbol GJB3
Gene ID (NCBI) 2707
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GJB3, also known as Connexin-31 (Cx31), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Mutations in the gene of GJB3 have been described in patients with dominant and recessive hearing impairment and in patients with erythrokeratodermia variabilis (EKV).

Protocols

Product Specific Protocols
WB protocol for GJB3 antibody 12880-1-APDownload protocol
IHC protocol for GJB3 antibody 12880-1-APDownload protocol
IF protocol for GJB3 antibody 12880-1-APDownload protocol
IP protocol for GJB3 antibody 12880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Genes Cells

Skp2 contributes to cell cycle progression in trophoblast stem cells and to placental development.

Authors - Yuhei Yamauchi
mouseIHC

J Pain Res

PMID: 29692624

Authors - Brice Bellessort
marmosetsIF

Sci Rep

Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea.

Authors - Makoto Hosoya
mouseIHC

Stem Cells

FBXL12-Mediated Degradation of ALDH3 Is Essential for Trophoblast Differentiation during Placental Development.

Authors - Masaaki Nishiyama
humanWB

Cell Rep

Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss.

Authors - Makoto Hosoya