• Featured Product
  • KD/KO Validated

GLRX2 Polyclonal antibody

GLRX2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 13381-1-AP

Synonyms

GLRX2, Glutaredoxin 2, Glutaredoxin 2, mitochondrial, GRX2



Tested Applications

Positive WB detected inHGC-27 cells, MCF-7 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13381-1-AP targets GLRX2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLRX2 fusion protein Ag4218
Full Name glutaredoxin 2
Calculated Molecular Weight 164 aa, 18 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC028113
Gene Symbol GLRX2
Gene ID (NCBI) 51022
RRIDAB_10643373
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLRX2(Glutaredoxin-2) is also named as GRX2 and belongs to the glutaredoxin family. It facilitates the maintenance of cellular redox homeostasis upon treatment with apoptotic agents, thereby preventing cardiolipin oxidation and cytochrome C release. The GLRX2, which exists in a dynamic equilibrium of enzymatically active monomers and quiescent dimers,is a 16-kDa protein directed either to the nucleus or to the mitochondria through differential splicing of the first exon(PMID: 17121859). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for GLRX2 antibody 13381-1-APDownload protocol
IF protocol for GLRX2 antibody 13381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Free Radic Biol Med

Compartmentally scavenging hepatic oxidants through AMPK/SIRT3-PGC1α axis improves mitochondrial biogenesis and glucose catabolism.

Authors - Meiling Wu
humanWB

Free Radic Biol Med

Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.

Authors - Kelei Dong
  • KD Validated
mouseWB

Int Immunopharmacol

Glutaredoxin 2 protects cardiomyocytes from hypoxia/reoxygenation-induced injury by suppressing apoptosis, oxidative stress, and inflammation via enhancing Nrf2 signaling.

Authors - Chengliang Li
mouseWB

Brain Res

Upregulation of Glutaredoxin 2 alleviates oxygen-glucose deprivation/reoxygenation-induced apoptosis and ROS production in neurons by enhancing Nrf2 signaling via modulation of GSK-3β.

Authors - Jian Wen
  • KD Validated
mouseWB

J Physiol Sci

Redox proteins are constitutively secreted by skeletal muscle.

Authors - Yasuko Manabe