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  • Featured Product
  • KD/KO Validated

GPR103 Polyclonal antibody

GPR103 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25247-1-AP

Synonyms

AQ27, G protein coupled receptor 103, GPR103, QRFPR, SP9155

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using GPR103 Polyclonal antibody (25247-1-AP)

    WB analysis of mouse brain using 25247-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25247-1-AP (GPR103 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using GPR103 Polyclonal antibody (25247-1-AP)

    IHC staining of human brain using 25247-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25247-1-AP (GPR103 antibody at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using GPR103 Polyclonal antibody (25247-1-AP)

    IHC staining of human brain using 25247-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25247-1-AP (GPR103 antibody at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25247-1-AP is GPR103 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR103 fusion protein Ag19048
Full Name pyroglutamylated RFamide peptide receptor
Calculated Molecular Weight 431 aa, 49 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC128133
Gene Symbol QRFPR
Gene ID (NCBI) 84109
RRIDAB_2879987
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GPR103 antibody 25247-1-APDownload protocol
IHC protocol for GPR103 antibody 25247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Neurochem

N-glycosylation of the human neuropeptide QRFP receptor (QRFPR) is essential for ligand binding and receptor activation.

Authors - Weiwei Wang
View Article
humanWB

J Clin Lab Anal

The variations in human orphan G protein-coupled receptor QRFPR affect PI3K-AKT-mTOR signaling.

Authors - Huanzheng Li
  • KD Validated
View Article