human brain tissue were subjected to SDS PAGE followed by western blot with 11994-1-AP(Glutamate receptor 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 11994-1-AP(Glutamate receptor 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11994-1-AP(Glutamate receptor 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11994-1-AP( Glutamate receptor 2 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11994-1-AP( Glutamate receptor 2 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Result of anti-Glutamate receptor 2 (IP:11994-1-AP, 4ug; Detection:11994-1-AP 1:500) with mouse brain tissue lysate 4000ug.

1X10^6 SH-SY5Y cells were stained with 0.2ug Glutamate receptor 2 antibody (11994-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.