Glutamate receptor 2 Polyclonal antibody
Glutamate receptor 2 Polyclonal Antibody for FC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 11994-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, human brain tissue, SKOV-3 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 20 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
11994-1-AP targets Glutamate receptor 2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Glutamate receptor 2 fusion protein Ag2662 |
Full Name | glutamate receptor, ionotropic, AMPA 2 |
Calculated molecular weight | 883 aa, 99 kDa |
Observed molecular weight | 94-105 kDa |
GenBank accession number | BC010574 |
Gene symbol | GRIA2 |
Gene ID (NCBI) | 2891 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA2 (glutamate receptor 2, GluR-2) belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels that mediate the fast component of excitatory postsynaptic currents in neurons of the central nervous system. These channels are assembled from 4 related subunits, GRIA1-4. The presence of GRIA2 in the heteromeric AMPA receptors impermeabilizes it to calcium, preventing possible calcium-mediated toxicity.
Protocols
Product Specific Protocols | |
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WB protocol for Glutamate receptor 2 antibody 11994-1-AP | Download protocol |
IHC protocol for Glutamate receptor 2 antibody 11994-1-AP | Download protocol |
IP protocol for Glutamate receptor 2 antibody 11994-1-AP | Download protocol |
FC protocol for Glutamate receptor 2 antibody 11994-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biochim Biophys Acta Mol Cell Res Behavioral and electrophysiological changes in female mice overexpressing ORAI1 in neurons. | ||
Mol Nutr Food Res As a Histone Deacetylase Inhibitor, γ-Aminobutyric Acid Upregulates GluR2 Expression: An In Vitro and In Vivo Study. | ||
Cell Death Dis Vezatin regulates seizures by controlling AMPAR-mediated synaptic activity. | ||
Neuroreport Mammalian target of rapamycin signaling pathway is involved in synaptic plasticity of the spinal dorsal horn and neuropathic pain in rats by regulating autophagy. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Lisa (Verified Customer) (10-04-2021) | The antibody signal is quite strong (50-70sec of exposure) and gives "clean" bands. The diluted antibody (TBS 0.1% Tween+5% BSA) does not work well anymore after 2-3 times of re-use
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