Glutamate receptor 2 Polyclonal antibody

Glutamate receptor 2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 11994-1-AP

Synonyms

GluA2, GluR 2, GluR B, GluR K2, GLUR2, GLURB, Glutamate receptor 2, GRIA2, HBGR2



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, SKOV-3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11994-1-AP targets Glutamate receptor 2 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glutamate receptor 2 fusion protein Ag2662
Full Name glutamate receptor, ionotropic, AMPA 2
Calculated molecular weight 883 aa, 99 kDa
Observed molecular weight 94-105 kDa
GenBank accession numberBC010574
Gene symbol GRIA2
Gene ID (NCBI) 2891
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA2 (glutamate receptor 2, GluR-2) belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels that mediate the fast component of excitatory postsynaptic currents in neurons of the central nervous system. These channels are assembled from 4 related subunits, GRIA1-4. The presence of GRIA2 in the heteromeric AMPA receptors impermeabilizes it to calcium, preventing possible calcium-mediated toxicity.

Protocols

Product Specific Protocols
WB protocol for Glutamate receptor 2 antibody 11994-1-APDownload protocol
IHC protocol for Glutamate receptor 2 antibody 11994-1-APDownload protocol
IP protocol for Glutamate receptor 2 antibody 11994-1-APDownload protocol
FC protocol for Glutamate receptor 2 antibody 11994-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochim Biophys Acta Mol Cell Res

Behavioral and electrophysiological changes in female mice overexpressing ORAI1 in neurons.

Authors - Filip Maciąg
mouseWB

J Neurosci

CAMK2-dependent signaling in neurons is essential for survival.

Authors - Martijn J Kool
WB

Mol Nutr Food Res

As a Histone Deacetylase Inhibitor, γ-Aminobutyric Acid Upregulates GluR2 Expression: An In Vitro and In Vivo Study.

Authors - Aiping Xing
mouseWB

Neurochem Res

Down-regulation of Pin1 in Temporal Lobe Epilepsy Patients and Mouse Model.

Authors - Lan Tang
mouseWB

Neural Regen Res

PMID: 31960817

Authors - Jie Zhang
ratWB

Mol Cell Neurosci

mGluR and NMDAR activation internalize distinct populations of AMPARs.

Authors - Casimiro Tanya M TM