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Glutamate receptor 2 Antibody 8 Publications

Rabbit Polyclonal| Catalog number: 11994-1-AP

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Con: 63 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, FC, ELISA
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Images See all

Glutamate receptor 2 Antibody WB mouse brain tissue 11994-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 11994-1-AP(Glutamate receptor 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Glutamate receptor 2 Antibody IP mouse brain tissue 11994-1-AP

IP Result of anti-Glutamate receptor 2 (IP:11994-1-AP, 4ug; Detection:11994-1-AP 1:500) with mouse brain tissue lysate 4000ug.

Glutamate receptor 2 Antibody IHC mouse brain tissue 11994-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 11994-1-AP( Glutamate receptor 2 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Glutamate receptor 2 Antibody FC SH-SY5Y cells 11994-1-AP

1X10^6 SH-SY5Y cells were stained with 0.2ug Glutamate receptor 2 antibody (11994-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue, human brain tissue, SKOV-3 cells
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
SH-SY5Y cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Glutamate receptor 2 fusion protein Ag2662
Full name:
glutamate receptor, ionotropic, AMPA 2
Calculated molecular weight:
883aa,99 kDa
Observed molecular weight:
94-105 kDa
GenBank accession number:
BC010574
Gene ID (NCBI):
2891
Gene symbol
GRIA2
Synonyms
GluA2, GluR 2, GluR B, GluR K2, GLUR2, GLURB, Glutamate receptor 2, GRIA2, HBGR2

Background

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA2 (glutamate receptor 2, GluR-2) belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels that mediate the fast component of excitatory postsynaptic currents in neurons of the central nervous system. These channels are assembled from 4 related subunits, GRIA1-4. The presence of GRIA2 in the heteromeric AMPA receptors impermeabilizes it to calcium, preventing possible calcium-mediated toxicity.


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