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GRPEL1 Polyclonal antibody

GRPEL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12720-1-AP

Synonyms

GREPEL1, GRPEL1, HMGE, Mt GrpE#1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using GRPEL1 Polyclonal antibody (12720-1-AP)

    WB analysis of MCF-7 using 12720-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12720-1-AP (GRPEL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GRPEL1 Polyclonal antibody (12720-1-AP)

    WB analysis using 12720-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12720-1-AP (GRPEL1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using GRPEL1 Polyclonal antibody (12720-1-AP)

    IP experiment of MCF-7 using 12720-1-AP

    IP result of anti-GRPEL1 (IP:12720-1-AP, 4ug; Detection:12720-1-AP 1:10000) with MCF-7 cells lysate 1000 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GRPEL1 Polyclonal antibody (12720-1-AP)

    IHC staining of human colon cancer using 12720-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12720-1-AP (GRPEL1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GRPEL1 Polyclonal antibody (12720-1-AP)

    IF Staining of HepG2 using 12720-1-AP

    Immunofluorescent analysis of HepG2 cells, using GRPEL1 antibody 12720-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293T cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

12720-1-AP targets GRPEL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRPEL1 fusion protein Ag3415
Full Name GrpE-like 1, mitochondrial (E. coli)
Calculated Molecular Weight 217 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC024242
Gene Symbol GRPEL1
Gene ID (NCBI) 80273
RRIDAB_2114331
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GRPEL1 antibody 12720-1-APDownload protocol
IHC protocol for GRPEL1 antibody 12720-1-APDownload protocol
IF protocol for GRPEL1 antibody 12720-1-APDownload protocol
IP protocol for GRPEL1 antibody 12720-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.

Authors - Chun-Shik Shin
View Article
humanWB

Nat Commun

Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy

Authors - Jonas Benjamin Michaelis
View Article
humanWB

EBioMedicine

The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis.

Authors - Pingbo Chen
View Article