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GSS Monoclonal antibody

GSS Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig, hamster and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F12G1

Cat no : 67598-1-Ig

Synonyms

Glutathione synthase, glutathione synthetase, GSH S, GSH synthetase, GSHS, GSS



Tested Applications

Positive WB detected inCHO cells, rat liver tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67598-1-Ig targets GSS in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, pig, hamster samples.

Tested Reactivity Human, Mouse, Rat, pig, hamster
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSS fusion protein Ag8354
Full Name glutathione synthetase
Calculated Molecular Weight 474 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC007927
Gene Symbol GSS
Gene ID (NCBI) 2937
RRIDAB_2882804
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GSS antibody 67598-1-IgDownload protocol
IHC protocol for GSS antibody 67598-1-IgDownload protocol
IF protocol for GSS antibody 67598-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity

Authors - Xiaohan Jin