|Positive WB detected in||HeLa cells, HEK-293 cells, K-562 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10390-1-AP targets HGS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||HGS fusion protein Ag0589|
|Full Name||hepatocyte growth factor-regulated tyrosine kinase substrate|
|Calculated molecular weight||86 kDa|
|Observed molecular weight||110-115 kDa|
|GenBank accession number||BC003565|
|Gene ID (NCBI)||9146|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting the function of STAM which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein schwannomin/merlin. The growth suppression activity of schwannomin/merlin requires HGS and the binding of schwannomin/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.
|Product Specific Protocols|
|WB protocol for HGS antibody 10390-1-AP||Download protocol|
|IHC protocol for HGS antibody 10390-1-AP||Download protocol|
|IF protocol for HGS antibody 10390-1-AP||Download protocol|
|IP protocol for HGS antibody 10390-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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