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  • Featured Product
  • KD/KO Validated

SPP Polyclonal antibody

SPP Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20416-1-AP

Synonyms

H13, hIMP1, histocompatibility (minor) 13, HM13, IMP 1, IMP1, IMPAS, IMPAS 1, Intramembrane protease 1, MSTP086, Presenilin like protein 3, PSENL3, PSL3, Signal peptide peptidase, SPP

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SPP Polyclonal antibody (20416-1-AP)

    WB analysis of HEK-293 using 20416-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SPP Polyclonal antibody (20416-1-AP)

    WB analysis of mouse kidney using 20416-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using SPP Polyclonal antibody (20416-1-AP)

    WB analysis of U2OS using 20416-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SPP Polyclonal antibody (20416-1-AP)

    WB analysis of mouse liver using 20416-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SPP Polyclonal antibody (20416-1-AP)

    IHC staining of human hepatocirrhosis using 20416-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SPP Polyclonal antibody (20416-1-AP)

    IHC staining of human hepatocirrhosis using 20416-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, mouse liver tissue, mouse kidney tissue, U2OS cells
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20416-1-AP is SPP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPP fusion protein Ag14158
Full Name histocompatibility (minor) 13
Calculated Molecular Weight 377 aa, 41 kDa
Observed Molecular Weight 45 kDa, 100 kDa
GenBank Accession NumberBC008938
Gene Symbol HM13
Gene ID (NCBI) 81502
RRIDAB_10665421
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SPP antibody 20416-1-APDownload protocol
IHC protocol for SPP antibody 20416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

PLoS One

Visualizing active enzyme complexes using a photoreactive inhibitor for proximity ligation--application on γ-secretase.

Authors - Schedin-Weiss Sophia S
View Article
humanIHC,WB

Cell Death Dis

Histocompatibility Minor 13 (HM13), targeted by miR-760, exerts oncogenic role in breast cancer by suppressing autophagy and activating PI3K-AKT-mTOR pathway

Authors - Haiyan Yang
  • KD Validated
View Article
humanWB

Front Pharmacol

Pan-cancer analysis suggests histocompatibility minor 13 is an unfavorable prognostic biomarker promoting cell proliferation, migration, and invasion in hepatocellular carcinoma

Authors - Jun Liu
  • KD Validated
View Article