|Positive WB detected in||HEK-293 cells, PC-3 cells, SH-SY5Y cells, C6 cells, NIH/3T3 cells|
|Positive IP detected in||mouse brain tissue|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Sample-dependent, check data in validation data gallery|
18295-1-AP targets HRAS-Specific in WB, IP, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||v-Ha-ras Harvey rat sarcoma viral oncogene homolog|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC006499|
|Gene ID (NCBI)||3265|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.
|Product Specific Protocols|
|WB protocol for HRAS-Specific antibody 18295-1-AP||Download protocol|
|IHC protocol for HRAS-Specific antibody 18295-1-AP||Download protocol|
|IP protocol for HRAS-Specific antibody 18295-1-AP||Download protocol|
|FC protocol for HRAS-Specific antibody 18295-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Benjamin (Verified Customer) (01-10-2020)
Identified HRAS around 20kDa mark on western blot.