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HRAS-Specific Polyclonal antibody (18295-1-AP)

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KD/KO Validated

HRAS-Specific Polyclonal antibody

HRAS-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 18295-1-AP

Publications(90)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

GTPase HRas, H RASIDX, HRAS, HRAS1, C BAS/HAS

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

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Freight/Packing: -

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Proteintech Guarantee

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 18295-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 18295-1-AP
WB result of HRAS-Specific antibody (18295-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRAS-Specific transfected HEK-293 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 18295-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 18295-1-AP
Recombinant proteins (ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS)) were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of PC-3 using 18295-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of SH-SY5Y using 18295-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 18295-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse brain using 18295-1-AP
IP result of anti-HRAS-Specific (IP:18295-1-AP, 4ug; Detection:18295-1-AP 1:1000) with mouse brain tissue lysate 1840 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HEK-293 using 18295-1-AP
IP result of anti-HRAS-Specific (IP:18295-1-AP, 4ug; Detection:18295-1-AP 1:1000) with HEK-293 cells lysate 1480 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 18295-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18295-1-AP (HRAS-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 18295-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HRAS-Specific antibody (18295-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 18295-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HRAS-Specific (18295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (90)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, SH-SY5Y cells, PC-3 cells, C6 cells, mouse kidney tissue, rat tissue, NIH/3T3 cells
Positive IP detected in	mouse brain tissue, HEK-293 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18295-1-AP targets HRAS-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Calculated Molecular Weight	21 kDa
Observed Molecular Weight	21 kDa
GenBank Accession Number	BC006499
Gene Symbol	HRAS
Gene ID (NCBI)	3265
RRID	AB_2121046
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P01112
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.

Protocols

Product Specific Protocols
FC protocol for HRAS-Specific antibody 18295-1-AP	Download protocol
IF protocol for HRAS-Specific antibody 18295-1-AP	Download protocol
IHC protocol for HRAS-Specific antibody 18295-1-AP	Download protocol
IP protocol for HRAS-Specific antibody 18295-1-AP	Download protocol
WB protocol for HRAS-Specific antibody 18295-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
mouse,human	WB	Nature Pan-KRAS inhibitor disables oncogenic signalling and tumour growth Authors - Dongsung Kim View Article
	WB	Cancer Cell Anticancer activity of RAS-GTP inhibition in cholangiocarcinoma Authors - Rodrigo Entrialgo-Cadierno View Article
human	WB	Nat Cancer Co-targeting SOS1 enhances the antitumor effects of KRASG12C inhibitors by addressing intrinsic and acquired resistance Authors - Venu Thatikonda View Article
human	WB	Nat Aging Aging-associated lncRNAs are evolutionarily conserved and participate in NFκB signaling Authors - Donghong Cai View Article
human	WB	Cancer Res Mutated HRAS Activates YAP1-AXL Signaling to Drive Metastasis of Head and Neck Cancer Authors - Sankar Jagadeeshan KD Validated View Article
		Cancer Res Oncogenic Transformation Drives DNA Methylation Loss and Transcriptional Activation of Transposable Element Loci Authors - Tomas Kanholm View Article

Reviews

4.5 out of 5

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2 verified customer reviews

What customers say

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Stella (Verified Customer) (08-26-2026)	Since the HRAS antibody from Santa Cruz failed, we have ordered your product for our publication (doi: 10.1038/s41419-026-08434-5). This was very successful for us and we have also published our blots using your antibody. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: CRC cell lines
Benjamin (Verified Customer) (01-10-2020)	Identified HRAS around 20kDa mark on western blot. Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: HEK