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  • KD/KO Validated

HRAS-Specific Polyclonal antibody

HRAS-Specific Polyclonal Antibody for FC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 18295-1-AP


C BAS/HAS, C H RAS, C HA RAS1, CTLO, GTPase HRas, H Ras 1, H RASIDX, Ha Ras, HAMSV, HRAS, HRAS1, K RAS, RASH1, Transforming protein p21

Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells, SH-SY5Y cells, C6 cells, NIH/3T3 cells
Positive IP detected inmouse brain tissue
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

18295-1-AP targets HRAS-Specific in WB, IP, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC006499
Gene symbol HRAS
Gene ID (NCBI) 3265
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.


Product Specific Protocols
WB protocol for HRAS-Specific antibody 18295-1-APDownload protocol
IHC protocol for HRAS-Specific antibody 18295-1-APDownload protocol
IP protocol for HRAS-Specific antibody 18295-1-APDownload protocol
FC protocol for HRAS-Specific antibody 18295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Agric Food Chem

Proteomic Analysis of Lysosomal Membrane Proteins in Bovine Mammary Epithelial Cells Illuminates Potential Novel Lysosome Functions in Lactation.

Authors - Chaochao Luo

Exp Ther Med

Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.

Authors - Min Li

Biochem Biophys Res Commun

Cell-type specific tumorigenesis with Ras oncogenes in human lung epithelial cells.

Authors - Minami Kumazaki

PLoS One

Eicosapentaenoic Acid Activates RAS/ERK/C/EBPβ Pathway through H-Ras Intron 1 CpG Island Demethylation in U937 Leukemia Cells.

Authors - Veronica Ceccarelli

Sci Rep

Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos.

Authors - Rosemary H C Wilson

Mol Carcinog

Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38 MAPK signaling pathways in human hepatoma HepG2 cells.

Authors - Wei Liu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Benjamin (Verified Customer) (01-10-2020)

Identified HRAS around 20kDa mark on western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK