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HRAS-Specific Polyclonal antibody
HRAS-Specific Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Cat no : 18295-1-AP
|Positive WB detected in||HEK-293 cells, PC-3 cells, SH-SY5Y cells, C6 cells, NIH/3T3 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human kidney tissue, human prostate hyperplasia tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
18295-1-AP targets HRAS-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||v-Ha-ras Harvey rat sarcoma viral oncogene homolog|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC006499|
|Gene ID (NCBI)||3265|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.
|Product Specific Protocols|
|WB protocol for HRAS-Specific antibody 18295-1-AP||Download protocol|
|IHC protocol for HRAS-Specific antibody 18295-1-AP||Download protocol|
|IP protocol for HRAS-Specific antibody 18295-1-AP||Download protocol|
|FC protocol for HRAS-Specific antibody 18295-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Agric Food Chem
Proteomic Analysis of Lysosomal Membrane Proteins in Bovine Mammary Epithelial Cells Illuminates Potential Novel Lysosome Functions in Lactation.
Exp Ther Med
Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.
Biochem Biophys Res Commun
Cell-type specific tumorigenesis with Ras oncogenes in human lung epithelial cells.
Eicosapentaenoic Acid Activates RAS/ERK/C/EBPβ Pathway through H-Ras Intron 1 CpG Island Demethylation in U937 Leukemia Cells.
Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos.
Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38 MAPK signaling pathways in human hepatoma HepG2 cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Benjamin (Verified Customer) (01-10-2020)
Identified HRAS around 20kDa mark on western blot.