Recombinant Human XCL1 protein (rFc Tag)
Species
Human
Purity
>80 %, SDS-PAGE
Tag
C-rFc
Activity
not tested
Cat no : Eg2933
Validation Data Gallery
Product Information
| Purity | >80 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human XCL1 protein Val22-Gly114 (Accession# P47992) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 6375 |
| Accession | P47992 |
| PredictedSize | 36.3 KDa |
| SDS-PAGE | |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
The human chemokine XCL1 is a metamorphic protein that exists as a mixture of two equally populated native state conformations under near-physiologic conditions. XCL1 interconverts spontaneously and reversibly between its two structures, which are (1) the canonical α − β chemokine fold, and (2) a 4-stranded β-sheet that forms a dimer.
References:
1. Tuinstra RL, et al. Proc. Natl. Acad. Sci. U. S. A 2008, 105, 5057. 2. Kuloglu ES, et al. Biochemistry 2001, 40, 12486. 3. Kuloglu ES, et al. J. Biol. Chem 2002, 277, 17863.