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- KD/KO Validated
IDH2 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
IHC, IP, WB, ELISA
Cat no : 15932-1-AP
ICD M, IDH, IDH2, IDHM, IDP, IDPM, mNADP IDH, NADP(+) specific ICDH, Oxalosuccinate decarboxylase
|Positive WB detected in||HEK-293 cells, mouse skeletal muscle tissue, Jurkat cells, mouse brain tissue, mouse heart tissue, rat liver tissue, SH-SY5Y cells, NIH/3T3 cells, HeLa cells, mouse liver tissue, HepG2 cells, K-562 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human colon cancer tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
15932-1-AP targets IDH2 in IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Reactivity||human, mouse, rat|
|Cited Species||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IDH2 fusion protein Ag8779|
|Full Name||isocitrate dehydrogenase 2 (NADP+), mitochondrial|
|Calculated molecular weight||452aa,51 kDa|
|Observed molecular weight||41-47 kDa|
|GenBank accession number||BC009244|
|Gene ID (NCBI)||3418|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.
J Mol Med (Berl)
Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.
Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.
Oxid Med Cell Longev
Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.
MiR-181a regulates lipid metabolism via IDH1.
Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.
On the Utility of Short Echo Time (TE) Single Voxel 1H-MRS in Non-Invasive Detection of 2-Hydroxyglutarate (2HG); Challenges and Potential Improvement Illustrated with Animal Models Using MRUI and LCModel.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree Sen (12-18-2019)
Product worked well in WB at 1:500 dilution