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IDH3G Polyclonal antibody

IDH3G Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 25848-1-AP

Synonyms

H IDHG, IDH3G

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using IDH3G Polyclonal antibody (25848-1-AP)

    WB analysis of mouse colon using 25848-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 25848-1-AP (IDH3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using IDH3G Polyclonal antibody (25848-1-AP)

    WB analysis of A431 using 25848-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25848-1-AP (IDH3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using IDH3G Polyclonal antibody (25848-1-AP)

    WB analysis of A375 using 25848-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 25848-1-AP (IDH3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IDH3G Polyclonal antibody (25848-1-AP)

    IHC staining of human skin cancer using 25848-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IDH3G Polyclonal antibody (25848-1-AP)

    IHC staining of human skin cancer using 25848-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using IDH3G Polyclonal antibody (25848-1-AP)

    IHC staining of human colon using 25848-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using IDH3G Polyclonal antibody (25848-1-AP)

    IHC staining of human colon using 25848-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse colon tissue, A375 cells, A431 cells
Positive IHC detected inhuman skin cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25848-1-AP is IDH3G Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IDH3G fusion protein Ag23113
Full Name isocitrate dehydrogenase 3 (NAD+) gamma
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC000933
Gene Symbol IDH3G
Gene ID (NCBI) 3421
RRIDAB_2880267
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IDH3G antibody 25848-1-APDownload protocol
IHC protocol for IDH3G antibody 25848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

Hypoxia-reprogrammed tricarboxylic acid cycle promotes the growth of human breast tumorigenic cells.

Authors - Ke Tang
View Article