IDH3G Polyclonal antibody

IDH3G Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25848-1-AP

Synonyms

H IDHG, IDH3G



Tested Applications

Positive WB detected inmouse colon tissue, A375 cells, A431 cells
Positive IHC detected inhuman skin cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25848-1-AP is IDH3G Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IDH3G fusion protein Ag23113
Full Name isocitrate dehydrogenase 3 (NAD+) gamma
Calculated molecular weight 43 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC000933
Gene symbol IDH3G
Gene ID (NCBI) 3421
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IDH3G antibody 25848-1-APDownload protocol
IHC protocol for IDH3G antibody 25848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

Hypoxia-reprogrammed tricarboxylic acid cycle promotes the growth of human breast tumorigenic cells.

Authors - Ke Tang