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IFITM2/3 Antibody 6 Publications

Mouse Monoclonal| Catalog number: 66081-1-Ig |CloneNo.: 2E8D12

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Con: 128 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

IFITM2/3 Antibody WB HeLa cells 66081-1-Ig

HeLa cells were subjected to SDS PAGE followed by western blot with 66081-1-Ig(IFITM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IFITM2/3 Antibody IHC human lung cancer tissue 66081-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung cancer using 66081-1-Ig(IFITM3 antibody) at dilution of 1:50 (under 10x lens)

IFITM2/3 Antibody IF HepG2 cells 66081-1-Ig

Immunofluorescent analysis of ( ) fixed HepG2 cells using 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:25 and N/A

IFITM2/3 Antibody FC HeLa cells 66081-1-Ig

1X10^6 HeLa cells were stained with 0.2ug IFITM2/3 antibody (66081-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Species specificity:
human
Positive WB detected in:
Hela cells, human placenta tissue
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IFITM2/3 fusion protein Ag17863
Full name:
interferon induced transmembrane protein 3 (1-8U)
Calculated molecular weight:
Observed molecular weight:
14 kDa
GenBank accession number:
BC070243
Gene ID (NCBI):
10410
Gene symbol
IFITM3
Synonyms
1 8U, IFITM3, IP15

Background

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.


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