• Featured Product
  • KD/KO Validated

IFT88 Polyclonal antibody

IFT88 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, dog and More (3)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13967-1-AP

Synonyms

IFT88, TPR repeat protein 10, TTC10



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, MDCK cells, NIH/3T3 cells
Positive IP detected inknockout cells and WT cells, HEK-293 cells
Positive IHC detected inhuman heart tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, hTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13967-1-AP targets IFT88 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, dog samples.

Tested Reactivity human, mouse, rat, dog
Cited Reactivity Chick, Chicken, dog, human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT88 fusion protein Ag4980
Full Name intraflagellar transport 88 homolog (Chlamydomonas)
Calculated molecular weight 94 kDa
Observed molecular weight 88-95 kDa
GenBank accession numberBC030776
Gene symbol IFT88
Gene ID (NCBI) 8100
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.

Protocols

Product Specific Protocols
WB protocol for IFT88 antibody 13967-1-APDownload protocol
IHC protocol for IFT88 antibody 13967-1-APDownload protocol
IF protocol for IFT88 antibody 13967-1-APDownload protocol
IP protocol for IFT88 antibody 13967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FASEB J

Failure to reabsorb the primary cilium induces cellular senescence.

Authors - Elizabeth P Jeffries
  • KD Validated
mouseIF

PLoS Genet

Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.

Authors - Emily Bowie
mouseIF

Dev Cell

Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation.

Authors - Chia-Hsiang Chang
  • KD Validated
humanIF

Am J Hum Genet

Bi-allelic Mutations in FAM149B1 Cause Abnormal Primary Cilium and a Range of Ciliopathy Phenotypes in Humans.

Authors - Ranad Shaheen
mouseWB,IF

Front Genet

661W Photoreceptor Cell Line as a Cell Model for Studying Retinal Ciliopathies.

Authors - Gabrielle Wheway
humanIF

Ann Neurol

Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.

Authors - Hyowon Hong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (01-31-2019)

Excellent. Works quite well both in WB and IF.

  • Applications: Western Blot,Immunofluorescence,