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IGF1A-Specific Polyclonal antibody

IGF1A-Specific Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20214-1-AP

Synonyms

IBP1, IGF I, IGF1, IGF-1, IGF1A, IGF1B, IGFI, Insulin like growth factor I, Mechano growth factor, MGF, Somatomedin C



Tested Applications

Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20214-1-AP targets IGF1A-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name insulin-like growth factor 1 (somatomedin C)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
Gene symbol IGF1
Gene ID (NCBI) 3479
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF1, also named as IBP1, MGF, IGF-IA and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but have a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. The antibody is specific to isoform IGF-1A.

Protocols

Product Specific Protocols
WB protocol for IGF1A-Specific antibody 20214-1-APDownload protocol
IHC protocol for IGF1A-Specific antibody 20214-1-APDownload protocol
IP protocol for IGF1A-Specific antibody 20214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Angle Orthod

Receptor activator of nuclear factor-kappa ligand, OPG, and IGF-I expression during orthodontically induced inflammatory root resorption in the recombinant human growth hormone-treated rats.

Authors - Yajun Hu

Neurosci Lett

AAV9-IGF1 protects TDP-25 cells from apoptosis and oxidative stress partly via up-regulating the expression of VEGF in vitro.

Authors - Zhongyao Li
humanWB

J Pharmacol Sci

Plumbagin suppresses the migration and invasion of glioma cells via downregulation of MMP-2/9 expression and inaction of PI3K/Akt signaling pathway in vitro.

Authors - Guanghui Chen
humanWB

Cancer Biol Ther

PMID: 31559898

Authors - Fan Wang
pigWB

PLoS One

Dietary protein-induced hepatic IGF-1 secretion mediated by PPARγ activation.

Authors - Xiaojuan Wan
mouseWB

Brain Res Bull

The role of insulin-like growth factor 1 in ALS cell and mouse models: a mitochondrial protector.

Authors - Di Wen
  • KO Validated