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IGF1A-Specific Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 20214-1-AP

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Con: 57 μg/150 μl

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Tested Applications
Applications:
ELISA, IF, IHC, IP, WB
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Images See all

IGF1A-Specific Antibody WB mouse testis tissue 20214-1-AP

mouse testis tissue were subjected to SDS PAGE followed by western blot with 20214-1-AP(IGF1A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

IGF1A-Specific Antibody IP mouse liver tissue 20214-1-AP

IP Result of anti-IGF1A-Specific (IP:20214-1-AP, 3ug; Detection:20214-1-AP 1:300) with mouse liver tissue lysate 5000ug.

IGF1A-Specific Antibody IHC human liver tissue 20214-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 20214-1-AP( IGF1A-Specific Antibody) at dilution of 1:200 (under 10x lens).

IGF1A-Specific Antibody IHC human liver tissue 20214-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 20214-1-AP( IGF1A-Specific Antibody) at dilution of 1:200 (under 40x lens).

IGF1A-Specific Antibody IF HepG2 cells 20214-1-AP

Immunofluorescent analysis of HepG2 cells using 20214-1-AP(IGF1A-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, pig, rat
Positive WB detected in:
mouse testis tissue, L02 cells, mouse liver tissue, mouse ovary tissue, mouse uterus tissue, PC-3 cells
Positive IP detected in:
mouse liver tissue
Positive IHC detected in:
human liver tissue, human liver tissue, human liver cancer tissue
Positive IF detected in:
HepG2 cells, HeLa cells, HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:200
IF : 1:20-1:200

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
insulin-like growth factor 1 (somatomedin C)
Calculated molecular weight:
17 kDa
Observed molecular weight:
15-20 kDa
GenBank accession number:
Gene ID (NCBI):
3479
Gene symbol
IGF1
Synonyms
IBP1, IGF I, IGF1, IGF1A, IGF1B, IGFI, Insulin like growth factor I, Mechano growth factor, MGF, Somatomedin C
Background

IGF1, also named as IBP1, MGF, IGF-IA and Somatomedin-C, belongs to the insulin family. IGF1 is structurally and functionally related to insulin but have a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. The antibody is specific to isoform IGF-1A.


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