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IGF1R beta chain Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 20254-1-AP

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Con: 33 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

IGF1R beta chain Antibody WB HeLa cells 20254-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 20254-1-AP(IGF1R beta chain antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

IGF1R beta chain Antibody IP HeLa cells 20254-1-AP

IP Result of anti-IGF1R beta chain (IP:20254-1-AP, 4ug; Detection:20254-1-AP 1:300) with HeLa cells lysate 2400ug.

IGF1R beta chain Antibody IHC human lung cancer tissue 20254-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 20254-1-AP( IGF1R beta chain antibody at dilution of 1:200 (under 10x lens).

IGF1R beta chain Antibody IHC human lung cancer tissue 20254-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 20254-1-AP( IGF1R beta chain antibody at dilution of 1:200 (under 40x lens).

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, L02 cells, MCF-7 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:100-1:400

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
insulin-like growth factor 1 receptor
Calculated molecular weight:
155 kDa
Observed molecular weight:
100 kDa
GenBank accession number:
NM_000875
Gene ID (NCBI):
3480
Gene symbol
IGF1R
Synonyms
CD221, IGF I receptor, IGF1R, IGFIR, IGFR, JTK13

Background

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cell. IGF1R is a heterotetrameric receptor, composed of two alpha subunits (130 kDa each) and two beta subunits (~98 kDa each) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds. This antibody raised against a synthesized polypeptide (CGVLVLRASFDERQPYAH) corresponding to 1332-1348aa of human IGF1R protein recognizes beta subunit of IGF1R.


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