|Positive WB detected in||HeLa cells, L02 cells, MCF-7 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
20254-1-AP targets IGF1R Beta Chain in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||IGF I receptor|
|Calculated molecular weight||155 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||NM_000875|
|Gene ID (NCBI)||3480|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cell. IGF1R is a heterotetrameric receptor, composed of two alpha subunits (130 kDa each) and two beta subunits (~98 kDa each) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds. This antibody raised against a synthesized polypeptide (CGVLVLRASFDERQPYAH) corresponding to 1332-1348aa of human IGF1R protein recognizes beta subunit of IGF1R.
|Product Specific Protocols|
|WB protocol for IGF1R Beta Chain antibody 20254-1-AP||Download protocol|
|IHC protocol for IGF1R Beta Chain antibody 20254-1-AP||Download protocol|
|IP protocol for IGF1R Beta Chain antibody 20254-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tongbin (Verified Customer) (08-25-2020)
This antibody had some faint non-specific bands in addition to a strong and specific IGF1R band.