- Featured Product
- KD/KO Validated
IGF1R Beta Chain Polyclonal antibody
IGF1R Beta Chain Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC,ELISA
Conjugate
Unconjugated
Cat no : 20254-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, L02 cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 22 publications below |
| IHC | See 5 publications below |
Product Information
20254-1-AP targets IGF1R Beta Chain in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | IGF I receptor |
| Calculated molecular weight | 155 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | NM_000875 |
| Gene symbol | IGF1R |
| Gene ID (NCBI) | 3480 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cell. IGF1R is a heterotetrameric receptor, composed of two alpha subunits (130 kDa each) and two beta subunits (~98 kDa each) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds. This antibody raised against a synthesized polypeptide (CGVLVLRASFDERQPYAH) corresponding to 1332-1348aa of human IGF1R protein recognizes beta subunit of IGF1R.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IGF1R Beta Chain antibody 20254-1-AP | Download protocol |
| IHC protocol for IGF1R Beta Chain antibody 20254-1-AP | Download protocol |
| IP protocol for IGF1R Beta Chain antibody 20254-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Transl Neurodegener Enriched gestation activates the IGF pathway to evoke embryo-adult benefits to prevent Alzheimer's disease. | ||
Ann Nutr Metab Decreased Amino Acid Concentrations are Involved in Congenital Heart Disease. | ||
Colloids Surf B Biointerfaces Toxicological exploration of peptide-based cationic liposomes in siRNA delivery.
| ||
Biochem Biophys Res Commun Lnc-SNHG1 may promote the progression of non-small cell lung cancer by acting as a sponge of miR-497. | ||
J Agric Food Chem Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tongbin (Verified Customer) (08-25-2020) | This antibody had some faint non-specific bands in addition to a strong and specific IGF1R band.
|
