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  • KD/KO Validated

IGF1R Beta Chain Polyclonal antibody

IGF1R Beta Chain Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20254-1-AP

Synonyms

CD221, IGF I receptor, IGF1R, IGFIR, IGFR, JTK13



Tested Applications

Positive WB detected inHeLa cells, L02 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

20254-1-AP targets IGF1R Beta Chain in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name IGF I receptor
Calculated molecular weight 155 kDa
Observed molecular weight 100 kDa
GenBank accession numberNM_000875
Gene symbol IGF1R
Gene ID (NCBI) 3480
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cell. IGF1R is a heterotetrameric receptor, composed of two alpha subunits (130 kDa each) and two beta subunits (~98 kDa each) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds. This antibody raised against a synthesized polypeptide (CGVLVLRASFDERQPYAH) corresponding to 1332-1348aa of human IGF1R protein recognizes beta subunit of IGF1R.

Protocols

Product Specific Protocols
WB protocol for IGF1R Beta Chain antibody 20254-1-APDownload protocol
IHC protocol for IGF1R Beta Chain antibody 20254-1-APDownload protocol
IP protocol for IGF1R Beta Chain antibody 20254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Transl Neurodegener

Enriched gestation activates the IGF pathway to evoke embryo-adult benefits to prevent Alzheimer's disease.

Authors - Enjie Liu
humanWB

Ann Nutr Metab

Decreased Amino Acid Concentrations are Involved in Congenital Heart Disease.

Authors - Guangrui Lai
humanWB

Colloids Surf B Biointerfaces

Toxicological exploration of peptide-based cationic liposomes in siRNA delivery.

Authors - Yanyan Zhu
  • KD Validated
humanWB

Biochem Biophys Res Commun

Lnc-SNHG1 may promote the progression of non-small cell lung cancer by acting as a sponge of miR-497.

Authors - Zhanxia Li
ratWB

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang
humanWB

Oncotarget

PMID: 28427228

Authors - Zhi-Hua Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (08-25-2020)

This antibody had some faint non-specific bands in addition to a strong and specific IGF1R band.

  • Applications: Western Blot
  • Cell Tissue Type: Heart