• Featured Product
  • KD/KO Validated

Mitofilin Antibody

Mitofilin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10179-1-AP

Synonyms

HMP, IMMT, MIC60, MINOS2, Mitofilin, P87, P87/89, P89, PIG4, PIG52



Tested Applications

Positive WB detected inmulti-cells/tissue, COLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, MCF-7 cells, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman gliomas tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10179-1-AP targets Mitofilin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity hamster, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Mitofilin fusion protein Ag0102
Full Name inner membrane protein, mitochondrial (mitofilin)
Calculated molecular weight 90 kDa
Observed molecular weight 87 kDa,89 kDa
GenBank accession number BC002412
Gene symbol IMMT
Gene ID (NCBI) 10989
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

Protocols

Product Specific Protocols
WB protocol for Mitofilin antibody 10179-1-APDownload protocol
IHC protocol for Mitofilin antibody 10179-1-APDownload protocol
IF protocol for Mitofilin antibody 10179-1-APDownload protocol
IP protocol for Mitofilin antibody 10179-1-APDownload protocol
FC protocol for Mitofilin antibody 10179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Genet

TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.

Authors - Peng Wang
human

Mol Cell

ER and Nutrient Stress Promote Assembly of Respiratory Chain Supercomplexes through the PERK-eIF2α Axis.

Authors - Eduardo Balsa
  • KO Validated
mouseWB

mSphere

Toxoplasma gondii MAF1b Binds the Host Cell MIB Complex To Mediate Mitochondrial Association.

Authors - Felice D Kelly
  • KD Validated
humanWB

Biochemistry

Pink1 forms a multiprotein complex with Miro and Milton, linking Pink1 function to mitochondrial trafficking.

Authors - Weihofen Andreas A
humanWB

PLoS One

Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV.

Authors - Rui Huang
humanWB

J Proteome Res

Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice.

Authors - Foteini Violitzi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

CYNTHIA (Verified Customer) (07-16-2020)

I used the mitofilin on my WB samples at the beginning it was great but in 3 times of using it i couldn't understand why it doesn't work. but i recommend ure antibodies to my colleagues

  • Applications: Western Blot
No image submitted with review
FH

Yan (Verified Customer) (01-28-2020)

This antibody worked very well on mouse heart tissue for western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue
No image submitted with review