ISCU Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 14812-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 44 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
mouse heart tissue, COLO 320 cells, HeLa cells, Raji cells

Positive IP detected in:
mouse heart tissue

Positive IHC detected in:
human lung cancer tissue, human endometrial cancer tissue

Positive IF detected in:
MCF-7 cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
iron-sulfur cluster scaffold homolog (E. coli)

Calculated molecular weight:
18 kDa

Observed molecular weight:
14-15 kDa,18-19 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ISCU

Synonyms
2310020H20Rik, HML, hnifU, ISCU, ISU2, NIFU, NifU like protein, NIFUN
Background

Iron-sulfur (Fe-S) clusters are required for the functions of mitochondrial aconitase, mammalian iron regulatory protein 1, and many other proteins in multiple subcellular compartments. Mitochondrial and cytosolic isoforms of the human Fe-S cluster scaffold protein, iron-sulfur cluster assembly enzyme (ISCU), is involved in activation of iron uptake, redistribution of intracellular iron, and decreased utilization of iron in Fe-S proteins.
Defects in ISCU gene are the cause of myopathy with exercise intolerance Swedish type (MEIS), which is characterized by lifelong severe exercise intolerance (PMID: 18304497). Two ISCU isoforms ISCU1 (cytosolic, 15 kDa) and ISCU2 (mitochondrial, 18 kDa), were produced by alternative splicing, and ISCU2 is considered the canonical isoform1. ISCU2 protein is rapidly processed upon maturation (PMID: 11060020). Proteintech’s ISCU antibody 14812-1-AP is raised against the full-length ISCU2 and is expected to detect 14 kDa,15 kDa and 18-19 kDa bands which has been reported in the previous observation (PMID: 11060020).


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