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CoraLite® Plus 488-conjugated ISCU Polyclonal antibody

ISCU Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG


human, mouse, rat




CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14812


2310020H20Rik, HML, hnifU, ISCU, ISCU2, ISU2, NIFU, NifU like protein, NIFUN

Tested Applications

Positive IF detected inMCF-7 cells

Recommended dilution

Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14812 targets ISCU in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ISCU fusion protein Ag6562
Full Name iron-sulfur cluster scaffold homolog (E. coli)
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 14-15 kDa, 18-19 kDa
GenBank Accession NumberBC061903
Gene Symbol ISCU
Gene ID (NCBI) 23479
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Iron-sulfur (Fe-S) clusters are required for the functions of mitochondrial aconitase, mammalian iron regulatory protein 1, and many other proteins in multiple subcellular compartments. Mitochondrial and cytosolic isoforms of the human Fe-S cluster scaffold protein, iron-sulfur cluster assembly enzyme (ISCU), is involved in activation of iron uptake, redistribution of intracellular iron, and decreased utilization of iron in Fe-S proteins.
Defects in ISCU gene are the cause of myopathy with exercise intolerance Swedish type (MEIS), which is characterized by lifelong severe exercise intolerance (PMID: 18304497). Two ISCU isoforms ISCU1 (cytosolic, 15 kDa) and ISCU2 (mitochondrial, 18 kDa), were produced by alternative splicing, and ISCU2 is considered the canonical isoform1. ISCU2 protein is rapidly processed upon maturation (PMID: 11060020). Proteintech's ISCU antibody 14812-1-AP is raised against the full-length ISCU2 and is expected to detect 14 kDa,15 kDa and 18-19 kDa bands which has been reported in the previous observation (PMID: 11060020).


Product Specific Protocols
IF protocol for CL Plus 488 ISCU antibody CL488-14812Download protocol
Standard Protocols
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