ITPA Polyclonal antibody

Name: ITPA Polyclonal antibody
Brand: Proteintech
SKU: 16134-1-AP

ITPA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16134-1-AP

Synonyms

C20orf37, HLC14 06 P, Inosine triphosphatase, ITPA, ITPase, OK/SW cl.9

Validation Data Gallery

Filter:

AllWBIPIHCIF

WB analysis of K-562 using 16134-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16134-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16134-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16134-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 16134-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16134-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16134-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16134-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16134-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16134-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16134-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 16134-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 16134-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 16134-1-AP
IP result of anti-ITPA (IP:16134-1-AP, 4ug; Detection:16134-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
IHC staining of human heart using 16134-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 16134-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse heart using 16134-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16134-1-AP (ITPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 16134-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 16134-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 16134-1-AP
Immunofluorescent analysis of MCF-7 cells, using ITPA antibody 11640-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 16134-1-AP

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Tested Applications

Positive WB detected in	K-562 cells, HeLa cells, HT-1080 cells, human liver tissue, mouse heart tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human heart tissue, human lung cancer tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below

Product Information

16134-1-AP targets ITPA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ITPA fusion protein Ag9128
Full Name	inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
Calculated Molecular Weight	194 aa, 21 kDa
Observed Molecular Weight	22 kDa
GenBank Accession Number	BC010138
Gene Symbol	ITPA
Gene ID (NCBI)	3704
RRID	AB_2129821
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITPA(Inosine triphosphate pyrophosphatase) catalyzes the pyrophosphohydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP).It expresses in the cytoplasm(PMID:11278832).It can be detected a band of 44 Kda as a homodimer in the western blot(PMID:19631656) though its predictedsize is 21kd.This protein has two isforms with the molecular weight of 20KDa and 21KDa.

Protocols

Product Specific Protocols
WB protocol for ITPA antibody 16134-1-AP	Download protocol
IHC protocol for ITPA antibody 16134-1-AP	Download protocol
IF protocol for ITPA antibody 16134-1-AP	Download protocol
IP protocol for ITPA antibody 16134-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Biochimie Analysis of human ITPase nucleobase specificity by site-directed mutagenesis. Authors - Hill Amanda A View Article
human	IHC	Oncotarget A four-gene signature predicts survival in clear-cell renal-cell carcinoma. Authors - Jun Dai View Article
human	WB	Biochim Biophys Acta Quantitative in vitro and in vivo characterization of the human P32T mutant ITPase. Authors - Herting Greg G View Article

ITPA Polyclonal antibody

ITPA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of K-562 using 16134-1-AP

WB analysis of mouse liver using 16134-1-AP

WB analysis of mouse liver using 16134-1-AP

WB analysis of mouse liver using 16134-1-AP

WB analysis of rat liver using 16134-1-AP

WB analysis of mouse heart using 16134-1-AP

WB analysis of human liver using 16134-1-AP

WB analysis of HeLa using 16134-1-AP

WB analysis of mouse heart using 16134-1-AP

WB analysis of K-562 using 16134-1-AP

WB analysis of K-562 using 16134-1-AP

WB analysis of HT-1080 using 16134-1-AP

WB analysis of NIH/3T3 using 16134-1-AP

IP experiment of mouse liver using 16134-1-AP

IHC staining of human heart using 16134-1-AP

IHC staining of human heart using 16134-1-AP

IHC staining of mouse heart using 16134-1-AP

IHC staining of human lung cancer using 16134-1-AP

IHC staining of human lung cancer using 16134-1-AP

IF Staining of MCF-7 using 16134-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Analysis of human ITPase nucleobase specificity by site-directed mutagenesis.

A four-gene signature predicts survival in clear-cell renal-cell carcinoma.

Quantitative in vitro and in vivo characterization of the human P32T mutant ITPase.