|Positive IHC detected in||human pancreas tissue, mouse pancreas tissue, rat pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human pancreas tissue, mouse pancreas tissue|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:10000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66198-1-Ig targets INS in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||INS fusion protein Ag8630|
|Calculated molecular weight||110aa,12 kDa|
|Observed molecular weight|
|GenBank accession number||BC005255|
|Gene ID (NCBI)||3630|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, pro-INS, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus. INS may also exist 36 kDa form corresponding to the hexameric INS form.
Cell Death Dis
Glutathione prevents chronic oscillating glucose intake-induced β-cell dedifferentiation and failure.
Diabetes Metab Syndr Obes
Chronic oscillating glucose challenges disarrange innate immune homeostasis to potentiate the variation of neutrophil-lymphocyte ratio in rats with or without hidden diabetes mellitus.
Biomol Ther (Seoul)
Involvement of Estrogen Receptor-α in the Activation of Nrf2-Antioxidative Signaling Pathways by Silibinin in Pancreatic β-Cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Vincent (Verified Customer) (07-08-2019)
This antibody offers a good detection of Insulin expression in both DM mice and WT mice. The insulin expression can be observed in the islet cells of pancreatic tissue.