Validation Data Gallery
|Positive IHC detected in||human pancreas tissue, mouse pancreas tissue, rat pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human pancreas tissue, mouse pancreas tissue|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:10000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66198-1-Ig targets INS in WB, IHC, IF, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||INS fusion protein Ag8630|
|Calculated molecular weight||110 aa, 12 kDa|
|GenBank accession number||BC005255|
|Gene ID (NCBI)||3630|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, pro-INS, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus. INS may also exist 36 kDa form corresponding to the hexameric INS form.
Metabolic effects of CCL5 deficiency in lean and obese mice
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Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice.
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Mitochondrial protein MPV17 promotes β-cell apoptosis in diabetogenesis
cGAS-STING mediates cytoplasmic mitochondrial-DNA-induced inflammatory signal transduction during accelerated senescence of pancreatic β-cells induced by metabolic stress.
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Green light exposure aggravates high-fat diet feeding-induced hepatic steatosis and pancreatic dysfunction in male mice.
J Cell Mol Med
SDF-1 inhibits the dedifferentiation of islet β cells in hyperglycaemia by up-regulating FoxO1 via binding to CXCR4.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Vincent (Verified Customer) (07-08-2019)
This antibody offers a good detection of Insulin expression in both DM mice and WT mice. The insulin expression can be observed in the islet cells of pancreatic tissue.