CoraLite®594-conjugated INS Monoclonal antibody

INS Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4B6A7

Cat no : CL594-66198

Synonyms

ILPR, INS, IRDN



Tested Applications

Positive IF detected inmouse pancreas tissue
Planning an IHC experiment? We recommend our IHCeasy Insulin Ready-To-Use IHC Kit. Insulin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66198-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL594-66198 targets INS in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen INS fusion protein Ag8630
Full Name INS
Calculated molecular weight 110 aa, 12 kDa
GenBank accession numberBC005255
Gene symbol INS
Gene ID (NCBI) 3630
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. It is synthesized as a precursor molecule, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus. INS may also exist 36 kDa form corresponding to the hexameric INS form.

Protocols

Product Specific Protocols
IF protocol for CL594 INS antibody CL594-66198Download protocol
Standard Protocols
Click here to view our Standard Protocols