JUNB Polyclonal antibody

JUNB Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10486-1-AP

Synonyms

AP 1, jun B proto oncogene, JUNB, Transcription factor jun B



Tested Applications

Positive WB detected inMCF7 cells, C6 cells, HeLa cells, HT-1080 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

10486-1-AP targets JUNB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JUNB fusion protein Ag0752
Full Name jun B proto-oncogene
Calculated molecular weight 36 kDa
Observed molecular weight 42 kDa, 43 kDa
GenBank accession numberBC004250
Gene symbol JUNB
Gene ID (NCBI) 3726
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context

Protocols

Product Specific Protocols
WB protocol for JUNB antibody 10486-1-APDownload protocol
IHC protocol for JUNB antibody 10486-1-APDownload protocol
IF protocol for JUNB antibody 10486-1-APDownload protocol
IP protocol for JUNB antibody 10486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Cancer Res

PMID: 31285951

Authors - Wen-Si Zhao
mouseWB

J Exp Med

USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.

Authors - Siyuan Chen