Gamma Catenin Polyclonal antibody

Gamma Catenin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11146-1-AP

Synonyms

γ-Catenin, ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, gamma Catenin, PDGB, PKGB



Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, HeLa cells, T-47D cells, A431 cells
Positive IHC detected inhuman pancreas cancer tissue, human breast cancer tissue, human colon cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

11146-1-AP targets Gamma Catenin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Gamma Catenin fusion protein Ag1627
Full Name junction plakoglobin
Calculated molecular weight 82 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC011865
Gene symbol JUP
Gene ID (NCBI) 3728
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.

Protocols

Product Specific Protocols
WB protocol for Gamma Catenin antibody 11146-1-APDownload protocol
IHC protocol for Gamma Catenin antibody 11146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochem Biophys Res Commun

Plakoglobin is involved in cytoskeletal rearrangement of podocytes under the regulation of UCH-L1.

Authors - Yili Fang
humanWB

PLoS One

Quantitative Proteomic Profiling the Molecular Signatures of Annexin A5 in Lung Squamous Carcinoma Cells.

Authors - Bing Sun
mouseWB

Oncogene

Loss of giant obscurins from breast epithelium promotes epithelial-to-mesenchymal transition, tumorigenicity and metastasis.

Authors - M Shriver
humanWB

J Clin Invest

The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.

Authors - Wanjin Li