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- KD/KO Validated
KAP1 Monoclonal antibody
KAP1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (2)
Applications
WB, IP, IHC, IF, ChIP, ELISA
Conjugate
Unconjugated
CloneNo.
1B9G12
Cat no : 66630-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IF | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
66630-1-Ig targets KAP1 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | KAP1 fusion protein Ag7519 |
| Full Name | tripartite motif-containing 28 |
| Calculated Molecular Weight | 89 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC004978 |
| Gene Symbol | TRIM28 |
| Gene ID (NCBI) | 10155 |
| RRID | AB_2732886 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. （PMID: 18590578 ）
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KAP1 antibody 66630-1-Ig | Download protocol |
| IHC protocol for KAP1 antibody 66630-1-Ig | Download protocol |
| IF protocol for KAP1 antibody 66630-1-Ig | Download protocol |
| IP protocol for KAP1 antibody 66630-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Epac1 activation by cAMP regulates cellular SUMOylation and promotes the formation of biomolecular condensates. | ||
Nucleic Acids Res SIRT6 coordinates with CHD4 to promote chromatin relaxation and DNA repair. | ||
BMC Pulm Med miR-125b-5p upregulation by TRIM28 induces cisplatin resistance in non-small cell lung cancer through CREB1 inhibition
| ||
Open Biol A critical threshold of MCM10 is required to maintain genome stability during differentiation of induced pluripotent stem cells into natural killer cells | ||
