c-Kit/CD117 Polyclonal antibody

c-Kit/CD117 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 18696-1-AP

Synonyms

C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit



Tested Applications

Positive WB detected inMCF7 cells, human brain tissue
Positive IHC detected instromal tumor tissue, human placenta tissue, human testis tissue, Insulinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18696-1-AP targets c-Kit/CD117 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Calculated molecular weight 110 kDa
Observed molecular weight 120 kDa, 145 kDa
GenBank accession numberBC071593
Gene symbol KIT
Gene ID (NCBI) 3815
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIT, also named as SCFR, c-Kit and CD117, is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. It is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand(SCF) binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Phosphorylation at tyrosine 721 of KIT allows binding and activation of PI3 kinase. Loss of expression of KIT appears to be associated with progression of some tumors (melanoma) and autocrine/paracrine stimulation of the kit/SCF system may participate in human solid tumors such as lung, breast, testicular and gynecological malignancies. Mutations in Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer.

Protocols

Product Specific Protocols
WB protocol for c-Kit/CD117 antibody 18696-1-APDownload protocol
IHC protocol for c-Kit/CD117 antibody 18696-1-APDownload protocol
IF protocol for c-Kit/CD117 antibody 18696-1-APDownload protocol
FC protocol for c-Kit/CD117 antibody 18696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
sheepIF

Vet Res Commun

RNA-Seq-based transcriptomic profiling of primary interstitial cells of Cajal in response to bovine viral diarrhea virus infection.

Authors - Shengnan Li
humanIHC

Leuk Res

The role of high serum CXCL13 level in Waldenström macroglobulinemia.

Authors - Yan-Yan Wu

Oncol Rep

Effects and mechanism of RhoC downregulation in suppressing ovarian cancer stem cell proliferation, drug resistance, invasion and metastasis.

Authors - Xiu-Bo Sang
humanWB

Tumour Biol

Orai1 mediates tumor-promoting store-operated Ca(2+) entry in human gastrointestinal stromal tumors via c-KIT and the extracellular signal-regulated kinase pathway.

Authors - Lei Wang
humanIF

J Exp Clin Cancer Res

Growth differentiation factor 15 contributes to marrow adipocyte remodeling in response to the growth of leukemic cells.

Authors - Wei Lu