|Positive WB detected in||MCF7 cells, human brain tissue|
|Positive IHC detected in||stromal tumor tissue, human placenta tissue, human testis tissue, Insulinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18696-1-AP targets c-Kit/CD117 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, sheep|
|Host / Isotype||Rabbit / IgG|
|Full Name||v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog|
|Calculated molecular weight||110 kDa|
|Observed molecular weight||120 kDa, 145 kDa|
|GenBank accession number||BC071593|
|Gene ID (NCBI)||3815|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
KIT, also named as SCFR, c-Kit and CD117, is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. It is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand(SCF) binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Phosphorylation at tyrosine 721 of KIT allows binding and activation of PI3 kinase. Loss of expression of KIT appears to be associated with progression of some tumors (melanoma) and autocrine/paracrine stimulation of the kit/SCF system may participate in human solid tumors such as lung, breast, testicular and gynecological malignancies. Mutations in Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer.
|Product Specific Protocols|
|WB protocol for c-Kit/CD117 antibody 18696-1-AP||Download protocol|
|IHC protocol for c-Kit/CD117 antibody 18696-1-AP||Download protocol|
|IF protocol for c-Kit/CD117 antibody 18696-1-AP||Download protocol|
|FC protocol for c-Kit/CD117 antibody 18696-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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