KLK3/PSA Polyclonal antibody

KLK3/PSA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10679-1-AP

Synonyms

APS, Gamma seminoprotein, hK3, Kallikrein 3, kallikrein related peptidase 3, KLK2A1, KLK3, P 30 antigen, Prostate specific antigen, PSA, Semenogelase, Seminin

Validation Data Gallery

  • WB analysis using 10679-1-AP

    WB analysis using 10679-1-AP

    DU 145(PSA-), PC-3(PSA-) and LNCaP (PSA+) cell lysates were subjected to SDS PAGE followed by western blot with 10679-1-AP (KLK3/PSA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 10679-1-AP

    IHC staining of human prostate cancer using 10679-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 10679-1-AP

    IHC staining of human prostate cancer using 10679-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, Transfected HEK-293 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

10679-1-AP targets KLK3/PSA in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLK3/PSA fusion protein Ag1060
Full Name kallikrein-related peptidase 3
Calculated molecular weight 29 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC005307
Gene symbol KLK3
Gene ID (NCBI) 354
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for KLK3/PSA antibody 10679-1-APDownload protocol
IHC protocol for KLK3/PSA antibody 10679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Med

Wnt5a induces and maintains prostate cancer cells dormancy in bone.

Authors - Dong Ren
View Article
IHC

J Cell Biochem

Identification of reciprocal microRNA-mRNA pairs associated with metastatic potential disparities in human prostate cancer cells and signaling pathway analysis.

Authors - Hui Ma
View Article
humanWB

Cancers (Basel)

KLF5 Is Crucial for Androgen-AR Signaling to Transactivate Genes and Promote Cell Proliferation in Prostate Cancer Cells.

Authors - Juan Li
View Article
humanWB

Mol Cancer Ther

PMID: 28802253

Authors - Linh N K Tran
View Article