KLK3/PSA Polyclonal antibody
KLK3/PSA Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC,ELISA
Conjugate
Unconjugated
Cat no : 10679-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, Transfected HEK-293 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 4 publications below |
| IHC | See 2 publications below |
Product Information
10679-1-AP targets KLK3/PSA in WB, IHC,ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLK3/PSA fusion protein Ag1060 |
| Full Name | kallikrein-related peptidase 3 |
| Calculated molecular weight | 29 kDa |
| Observed molecular weight | 30-34 kDa |
| GenBank accession number | BC005307 |
| Gene symbol | KLK3 |
| Gene ID (NCBI) | 354 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KLK3/PSA antibody 10679-1-AP | Download protocol |
| IHC protocol for KLK3/PSA antibody 10679-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biochem Identification of reciprocal microRNA-mRNA pairs associated with metastatic potential disparities in human prostate cancer cells and signaling pathway analysis. | ||
Cancers (Basel) KLF5 Is Crucial for Androgen-AR Signaling to Transactivate Genes and Promote Cell Proliferation in Prostate Cancer Cells. | ||
Int J Biol Sci PRPF6 promotes androgen receptor/androgen receptor-variant 7 actions in castration-resistant prostate cancer cells. | ||
Theranostics ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3. |
