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KLK3/PSA Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 10679-1-AP

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

KLK3/PSA Antibody WB LNCaP cells 10679-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10679-1-AP (KLK3/PSA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

KLK3/PSA Antibody IHC human prostate cancer tissue 10679-1-AP

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP( KLK3/PSA antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

KLK3/PSA Antibody IHC human prostate cancer tissue 10679-1-AP

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP( KLK3/PSA antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human
Positive WB detected in:
LNCaP cells, Transfected HEK-293 cells
Positive IHC detected in:
human prostate cancer tissue
Recommended dilution:
WB : 1:1000-1:8000
IHC : 1:100-1:400

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
KLK3/PSA fusion protein Ag1060
Full name:
kallikrein-related peptidase 3
Calculated molecular weight:
29 kDa
Observed molecular weight:
31 kDa
GenBank accession number:
BC005307
Gene ID (NCBI):
354
Gene symbol
KLK3
Synonyms
APS, Gamma seminoprotein, hK3, Kallikrein 3, kallikrein related peptidase 3, KLK2A1, KLK3, P 30 antigen, Prostate specific antigen, PSA, Semenogelase, Seminin

Background

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms produced by alternative splicing.


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