Back

KMO Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 10698-1-AP

  • Print page
  • Download PDF

Be the first to review this product

-_-

Freight/Packing

Con: 39 μg/150 μl

Choose size:

Please visit your regions distributor:

Request quote for
bulk/custom formulations

Related Primary Antibodies
Special offer: Add control/tag antibody for $99
Load more

Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

KMO Antibody WB mouse heart tissue 10698-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 10698-1-AP(KMO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

KMO Antibody IHC human kidney tissue 10698-1-AP

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 10698-1-AP( KMO Antibody) at dilution of 1:200 (under 10x lens).

KMO Antibody IHC human kidney tissue 10698-1-AP

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 10698-1-AP( KMO Antibody) at dilution of 1:200 (under 40x lens).

KMO Antibody IF HepG2 cells 10698-1-AP

Immunofluorescent analysis of HepG2 cells using 10698-1-AP(KMO antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse heart tissue, HeLa cells, mouse spleen tissue, rat spleen tissue
Positive IHC detected in:
human kidney tissue, human prostate cancer tissue, human breast cancer tissue, mouse heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, MCF-7 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
KMO fusion protein Ag1109
Full name:
kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
Calculated molecular weight:
55.8 kDa
Observed molecular weight:
56 kDa
GenBank accession number:
BC005297
Gene ID (NCBI):
8564
Gene symbol
KMO
Synonyms
KMO, Kynurenine 3 hydroxylase, Kynurenine 3 monooxygenase

Background

KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-tryptophan metabolite L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).


Back
to top