KMO Polyclonal antibody
KMO Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10698-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, HeLa cells, mouse spleen tissue, rat spleen tissue |
| Positive IHC detected in | human kidney tissue, human prostate cancer tissue, human breast cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 7 publications below |
| IHC | See 5 publications below |
| IF | See 5 publications below |
Product Information
10698-1-AP targets KMO in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KMO fusion protein Ag1109 |
| Full Name | kynurenine 3-monooxygenase (kynurenine 3-hydroxylase) |
| Calculated molecular weight | 486 aa, 56 kDa |
| Observed molecular weight | 56 kDa |
| GenBank accession number | BC005297 |
| Gene symbol | KMO |
| Gene ID (NCBI) | 8564 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KMO antibody 10698-1-AP | Download protocol |
| IHC protocol for KMO antibody 10698-1-AP | Download protocol |
| IF protocol for KMO antibody 10698-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Med Rep Fr‑HMGB1 and ds‑HMGB1 activate the kynurenine pathway via different mechanisms in association with depressive‑like behavior. | ||
J Oncol Overexpression of Kynurenine 3-Monooxygenase Correlates with Cancer Malignancy and Predicts Poor Prognosis in Canine Mammary Gland Tumors. | ||
Vet Comp Oncol Kynurenine 3-Monooxygenase (KMO), and Signal Transducer and Activator of Transcription 3 (STAT3) Expression is involved in Tumor Proliferation and Predicts Poor Survival in Canine Melanoma. | ||
Mol Imaging Tryptophan PET Imaging of the Kynurenine Pathway in Patient-Derived Xenograft Models of Glioblastoma. | ||
J Biomol Screen Development of LC/MS/MS, High-Throughput Enzymatic and Cellular Assays for the Characterization of Compounds That Inhibit Kynurenine Monooxygenase (KMO). |
