KMO Polyclonal antibody

KMO Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10698-1-AP

Synonyms

KMO, Kynurenine 3 hydroxylase, Kynurenine 3 monooxygenase



Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, mouse spleen tissue, rat spleen tissue
Positive IHC detected inhuman kidney tissue, human prostate cancer tissue, human breast cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10698-1-AP targets KMO in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KMO fusion protein Ag1109
Full Name kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
Calculated molecular weight486 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC005297
Gene symbol KMO
Gene ID (NCBI) 8564
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).

Protocols

Product Specific Protocols
WB protocol for KMO antibody 10698-1-APDownload protocol
IHC protocol for KMO antibody 10698-1-APDownload protocol
IF protocol for KMO antibody 10698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Med Rep

Fr‑HMGB1 and ds‑HMGB1 activate the kynurenine pathway via different mechanisms in association with depressive‑like behavior.

Authors - Bo Wang
humanWB,IHC

J Oncol

Overexpression of Kynurenine 3-Monooxygenase Correlates with Cancer Malignancy and Predicts Poor Prognosis in Canine Mammary Gland Tumors.

Authors - Yi-Han Chiu
humanIHC

Vet Comp Oncol

Kynurenine 3-Monooxygenase (KMO), and Signal Transducer and Activator of Transcription 3 (STAT3) Expression is involved in Tumor Proliferation and Predicts Poor Survival in Canine Melanoma.

Authors - I-Li Liu
human,mouseIHC

Mol Imaging

Tryptophan PET Imaging of the Kynurenine Pathway in Patient-Derived Xenograft Models of Glioblastoma.

Authors - Anthony R Guastella
humanIF

J Biomol Screen

Development of LC/MS/MS, High-Throughput Enzymatic and Cellular Assays for the Characterization of Compounds That Inhibit Kynurenine Monooxygenase (KMO).

Authors - Winkler Dirk D
ratWB,IHC

Brain Res

PMID: 29800553

Authors - M M Guixia Xu