Cytokeratin 1-specific Polyclonal antibody

Cytokeratin 1-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16848-1-AP

Synonyms

67 kDa cytokeratin, CK 1, CK1, Cytokeratin 1, keratin 1, KRT1



Tested Applications

Positive WB detected inmouse skin tissue, A375 cells, A431 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman skin tissue, human cervix tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16848-1-AP targets Cytokeratin 1-specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity goat, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name keratin 1
Calculated molecular weight 66 kDa
Observed molecular weight 67 kDa
GenBank accession numberNM_006121
Gene symbol KRT1
Gene ID (NCBI) 3848
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. This antibody is specifically against ketatin I.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 1-specific antibody 16848-1-APDownload protocol
IHC protocol for Cytokeratin 1-specific antibody 16848-1-APDownload protocol
IF protocol for Cytokeratin 1-specific antibody 16848-1-APDownload protocol
IP protocol for Cytokeratin 1-specific antibody 16848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Ther Med

Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.

Authors - Min Li
goatWB

Animals (Basel)

Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications.

Authors - Sen Ma
mouseIHC

Life Sci

The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model.

Authors - Shidi Wu
humanIF

Sci Rep

Loss-of-function Mutation in PMVK Causes Autosomal Dominant Disseminated Superficial Porokeratosis.

Authors - Jiuxiang Wang