Cytokeratin 20 Antibody
Cytokeratin 20 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 17329-1-AP
Synonyms
CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT
Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, HeLa cells |
| Positive IHC detected in | human colon tissue, human stomach cancer tissue, human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2500-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:6400 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
17329-1-AP targets Cytokeratin 20 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cytokeratin 20 fusion protein Ag11308 |
| Full Name | keratin 20 |
| Calculated molecular weight | 424aa,48 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC031559 |
| Gene symbol | KRT20 |
| Gene ID (NCBI) | 54474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 20 antibody 17329-1-AP | Download protocol |
| IHC protocol for Cytokeratin 20 antibody 17329-1-AP | Download protocol |
| IF protocol for Cytokeratin 20 antibody 17329-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Immunol IL-13 secreted by ILC2s promotes the self-renewal of intestinal stem cells through circular RNA circPan3. | ||
PLoS One Urothelial Defects from Targeted Inactivation of Exocyst Sec10 in Mice Cause Ureteropelvic Junction Obstructions. | ||
Mol Cancer DC - SIGNR by influencing the lncRNA HNRNPKP2 upregulates the expression of CXCR4 in gastric cancer liver metastasis. | ||
Biomaterials A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium. | ||
Nat Cell Biol LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis. |
