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Cytokeratin 20 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 17329-1-AP

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Cytokeratin 20 Antibody WB COLO 320 cells 17329-1-AP

COLO 320 cells were subjected to SDS PAGE followed by western blot with 17329-1-AP( Cytokeratin 20 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours

Cytokeratin 20 Antibody IHC human colon tissue 17329-1-AP

Immunohistochemistry of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:6400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Cytokeratin 20 Antibody IHC human colon tissue 17329-1-AP

Immunohistochemistry of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:6400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Cytokeratin 20 Antibody IF HeLa cells 17329-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 17329-1-AP(Cytokeratin 20 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
COLO 320 cells, HeLa cells
Positive IHC detected in:
human colon tissue, human stomach cancer tissue, human urothelial carcinoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:2500-1:10000
IHC : 1:1000-1:6400
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Cytokeratin 20 fusion protein Ag11308
Full name:
keratin 20
Calculated molecular weight:
424aa,48 kDa
Observed molecular weight:
46 kDa
GenBank accession number:
BC031559
Gene ID (NCBI):
54474
Gene symbol
KRT20
Synonyms
CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT

Background

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.


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