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Cytokeratin 20 Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 17329-1-AP

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Cytokeratin 20 Antibody WB COLO 320 cells 17329-1-AP

COLO 320 cells were subjected to SDS PAGE followed by western blot with 17329-1-AP( Cytokeratin 20 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours

Cytokeratin 20 Antibody IHC human colon tissue 17329-1-AP

Immunohistochemistry of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:6400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Cytokeratin 20 Antibody IHC human colon tissue 17329-1-AP

Immunohistochemistry of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:6400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Cytokeratin 20 Antibody IF HeLa cells 17329-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 17329-1-AP(Cytokeratin 20 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
COLO 320 cells, HeLa cells
Positive IHC detected in:
human colon tissue, human stomach cancer tissue, human urothelial carcinoma tissue, rat bladder tissue, human ovary tumor tissue, human appendix tissue, rat colon tissue, human colon cancer tissue
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:2500-1:10000
IHC : 1:1000-1:6400
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Cytokeratin 20 fusion protein Ag11308
Full name:
keratin 20
Calculated molecular weight:
424aa,48 kDa
Observed molecular weight:
46 kDa
GenBank accession number:
BC031559
Gene ID (NCBI):
54474
Gene symbol
KRT20
Synonyms
CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT

Background

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.


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