CoraLite® Plus 488-conjugated Cytokeratin 20 Polyclonal antibody

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  IF Staining of HT-29 using CL488-17329

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-17329) at dilution of 1:200.

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  FC experiment of HT-29 using CL488-17329

  1x10^6 HT-29 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Cytokeratin 20 Polyclonal antibody (CL488-17329)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Cytokeratin 20 Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-17329

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, rabbit

Applications

IF/ICC, FC (Intra)

Synonyms

KRT20, CK 20, CK20, CK-20, Cytokeratin-20

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Tested Applications

• Positive IF/ICC detected in: HT-29 cells
• Positive FC (Intra) detected in: HT-29 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17329 targets Cytokeratin 20 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, rabbit samples.

• Tested Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11308

  Product name: Recombinant human KRT20 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 74-424 aa of BC031559

  Sequence: MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI

• Full Name: keratin 20
• Calculated Molecular Weight: 424 aa, 48 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC031559
• Gene Symbol: Cytokeratin 20
• Gene ID (NCBI): 54474
• RRID: AB_3084003
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35900
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.