Tested Applications
| Positive WB detected in | A431 cells, AML12 cells, HeLa cells, human saliva, mouse skin tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human skin cancer tissue, human bowen disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16853-1-AP targets Cytokeratin 6A-specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | keratin 6A |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | NM_005554 |
| Gene Symbol | Cytokeratin 6A |
| Gene ID (NCBI) | 3853 |
| RRID | AB_2134304 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02538 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence. This antibody is against all three isoforms of keratin 6, but it does not bind other keratins.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Cytokeratin 6A-specific antibody 16853-1-AP | Download protocol |
| IP protocol for Cytokeratin 6A-specific antibody 16853-1-AP | Download protocol |
| WB protocol for Cytokeratin 6A-specific antibody 16853-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cytokeratin 6A-specific Polyclonal antibody (Cat# 16853-1-AP) has 1 citation. It appears in Stem Cell Research & Therapy (IF 7.8), a 2026 study investigating how mesenchymal stem cells alleviate psoriasis by regulating the PSPH-PINK1-Parkin-NLRP3 pathway in HaCaT cells. The research field spans serine metabolism, stem cell therapy, and inflammatory skin disease. The antibody was used for WB and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Stem Cell Res Ther Research based on serine metabolism indicates mesenchymal stem cells alleviate psoriasis by regulating the PSPH-PINK1-Parkin-NLRP3 pathway in HaCaT. |
