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  • KD/KO Validated

KTN1 Polyclonal antibody

KTN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19841-1-AP

Synonyms

CG 1 antigen, CG1, KIAA0004, Kinectin, kinectin 1 (kinesin receptor), Kinesin receptor, KNT, KTN1, MU RMS 40.19



Tested Applications

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, K-562 cells, PC-3 cells, SKOV-3 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman ovary tumor tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19841-1-AP targets KTN1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KTN1 fusion protein Ag13854
Full Name kinectin 1 (kinesin receptor)
Calculated Molecular Weight 1357 aa, 156 kDa
Observed Molecular Weight 156 kDa
GenBank Accession NumberBC117132
Gene Symbol KTN1
Gene ID (NCBI) 3895
RRIDAB_10642436
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KTN1/Kinectin is an integral endoplasmic reticulum (ER) membrane protein which acts as dynamic anchor to facilitate ER membrane extension along microtubules. It has been found to bind kinesin, Rho GTPase, and translation elongation factor-1delta and gets involved in organelle motility and protein synthesis.

Protocols

Product Specific Protocols
WB protocol for KTN1 antibody 19841-1-APDownload protocol
IHC protocol for KTN1 antibody 19841-1-APDownload protocol
IF protocol for KTN1 antibody 19841-1-APDownload protocol
IP protocol for KTN1 antibody 19841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,monkeyWB

Nature

ER proteins decipher the tubulin code to regulate organelle distribution

Authors - Pengli Zheng
  • KO Validated
human,mouseWB

Sci Transl Med

CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease.

Authors - Sara Picó
humanIF

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
IF

EMBO Rep

Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.

Authors - Menno Spits
humanWB, IHC

Clin Sci (Lond)

The long noncoding RNA KTN1-AS1 promotes bladder cancer tumorigenesis via KTN1 cis-activation and the consequent initiation of Rho GTPase-mediated signaling.

Authors - Xueying Hu
monkeyWB

Curr Biol

ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.

Authors - Qingzhou Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Siting (Verified Customer) (01-28-2020)

This antibody worked well with western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue