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- KD/KO Validated
KU70,XRCC6 Polyclonal antibody
KU70,XRCC6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10723-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, MCF-7 cells, mouse colon tissue, A549 cells, Hela cells, mouse spleen tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, mouse kidney tissue, rat testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
10723-1-AP targets KU70,XRCC6 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KU70,XRCC6 fusion protein Ag1043|
|Full Name||X-ray repair complementing defective repair in Chinese hamster cells 6|
|Calculated molecular weight||75 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC008343|
|Gene ID (NCBI)||2547|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.
|Product Specific Protocols|
|WB protocol for KU70,XRCC6 antibody 10723-1-AP||Download protocol|
|IHC protocol for KU70,XRCC6 antibody 10723-1-AP||Download protocol|
|IF protocol for KU70,XRCC6 antibody 10723-1-AP||Download protocol|
|IP protocol for KU70,XRCC6 antibody 10723-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jonathan (Verified Customer) (02-03-2019)
Worked well for Western Blotting with little background.