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  • KD/KO Validated

KU70 Polyclonal antibody

KU70 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10723-1-AP

Synonyms

5 dRP lyase Ku70, 70 kDa subunit of Ku antigen, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, KU70, ML8, Thyroid lupus autoantigen, TLAA, XRCC6



Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, mouse colon tissue, A549 cells, Hela cells, mouse spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, mouse kidney tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10723-1-AP targets KU70 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KU70 fusion protein Ag1043
Full Name X-ray repair complementing defective repair in Chinese hamster cells 6
Calculated molecular weight 75 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC008343
Gene symbol XRCC6
Gene ID (NCBI) 2547
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

Protocols

Product Specific Protocols
WB protocol for KU70 antibody 10723-1-APDownload protocol
IHC protocol for KU70 antibody 10723-1-APDownload protocol
IF protocol for KU70 antibody 10723-1-APDownload protocol
IP protocol for KU70 antibody 10723-1-APDownload protocol
FC protocol for KU70 antibody 10723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Oncogene

UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.

Authors - L Hu
ratWB

FASEB J

Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats.

Authors - Catherine E Aiken
humanWB

Front Oncol

ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway

Authors - Xin Wen
humanWB

iScience

NRBF2 regulates the chemoresistance of small cell lung cancer by interacting with the P62 protein in the autophagy process.

Authors - Weitao Shen
humanWB,CoIP

iScience

WASH interacts with Ku to regulate DNA double-stranded break repair.

Authors - Tao Wang
  • KD Validated
humanIP

J Biol Chem

Epithelial Cell Transforming Factor ECT2 Is an Important Regulator of DNA Double-Strand Break Repair and Genome Stability.

Authors - Cheng Cao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jonathan (Verified Customer) (02-03-2019)

Worked well for Western Blotting with little background.

  • Applications: Western Blot,