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KU70 Polyclonal antibody
KU70 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10723-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, MCF-7 cells, A549 cells, Hela cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, mouse kidney tissue, rat testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10723-1-AP targets KU70 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KU70 fusion protein Ag1043|
|Full Name||X-ray repair complementing defective repair in Chinese hamster cells 6|
|Calculated molecular weight||75 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC008343|
|Gene ID (NCBI)||2547|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.
|Product Specific Protocols|
|WB protocol for KU70 antibody 10723-1-AP||Download protocol|
|IHC protocol for KU70 antibody 10723-1-AP||Download protocol|
|IF protocol for KU70 antibody 10723-1-AP||Download protocol|
|IP protocol for KU70 antibody 10723-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats.
Cell Death Dis
LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma.
Cell Death Dis
OGA is associated with deglycosylation of NONO and the KU complex during DNA damage repair.
Cell Death Dis
Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca2+]m-PDP1-PDH-histone acetylation retrograde signaling.
Int J Radiat Biol
RNAi silencing targeting RNF8 enhances radiosensitivity of a non-small cell lung cancer cell line A549.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jonathan (Verified Customer) (02-03-2019)
Worked well for Western Blotting with little background.