• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

LACTB2 Polyclonal antibody

LACTB2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16783-1-AP

Synonyms

Beta lactamase like protein 2, CGI 83, lactamase, beta 2, LACTB2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A549 cells using LACTB2 Polyclonal antibody (16783-1-AP)

    WB analysis of A549 using 16783-1-AP

    WB result of LACTB2 antibody (16783-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LACTB2 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using LACTB2 Polyclonal antibody (16783-1-AP)

    WB analysis using 16783-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using LACTB2 Polyclonal antibody (16783-1-AP)

    WB analysis of PC-3 using 16783-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using LACTB2 Polyclonal antibody (16783-1-AP)

    WB analysis of Y79 using 16783-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using LACTB2 Polyclonal antibody (16783-1-AP)

    IP experiment of A549 using 16783-1-AP

    IP result of anti-LACTB2 (IP:16783-1-AP, 3ug; Detection:16783-1-AP 1:700) with A549 cells lysate 520ug.

  • Immunohistochemistry (IHC) staining of human cervix tissue using LACTB2 Polyclonal antibody (16783-1-AP)

    IHC staining of human cervix using 16783-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 16783-1-AP (LACTB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using LACTB2 Polyclonal antibody (16783-1-AP)

    IHC staining of human cervix using 16783-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 16783-1-AP (LACTB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LACTB2 Polyclonal antibody (16783-1-AP)

    IF Staining of HepG2 using 16783-1-AP

    Immunofluorescent analysis of HepG2 cells, using LACTB2 antibody 16783-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inMCF-7 cells, PC-3 cells, Y79 cells, A549 cells, HeLa cells
Positive IP detected inA549 cells
Positive IHC detected inhuman cervix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16783-1-AP targets LACTB2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LACTB2 fusion protein Ag10213
Full Name lactamase, beta 2
Calculated Molecular Weight 288 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC000878
Gene Symbol LACTB2
Gene ID (NCBI) 51110
RRIDAB_2133333
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LACTB2 antibody 16783-1-APDownload protocol
IHC protocol for LACTB2 antibody 16783-1-APDownload protocol
IF protocol for LACTB2 antibody 16783-1-APDownload protocol
IP protocol for LACTB2 antibody 16783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols