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LCOR Polyclonal antibody

LCOR Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14476-1-AP

Synonyms

KIAA1795, LCOR, Ligand dependent corepressor, Mblk1 related protein 2, MLR2, RP11 175O19.1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LCOR Polyclonal antibody (14476-1-AP)

    WB analysis of HeLa using 14476-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14476-1-AP (LCOR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using LCOR Polyclonal antibody (14476-1-AP)

    IHC staining of human endometrial cancer using 14476-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14476-1-AP (LCOR antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using LCOR Polyclonal antibody (14476-1-AP)

    IHC staining of human endometrial cancer using 14476-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14476-1-AP (LCOR antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LCOR Polyclonal antibody (14476-1-AP)

    IF Staining of HeLa using 14476-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14476-1-AP (LCOR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14476-1-AP targets LCOR in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LCOR fusion protein Ag5851
Full Name ligand dependent nuclear receptor corepressor
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC053359
Gene Symbol LCOR
Gene ID (NCBI) 84458
RRIDAB_2136862
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LCOR is a transcriptional corepressor widely expressed in fetal and adult tissues that is recruited to agonist-bound nuclear receptors through a single LxxLL motif, also referred to as a nuclear receptor (NR) box [PMID:12535528]. It functions through recruitment of C-terminal binding proteins (CtBPs) and histone deacetylases (HDACs) to its N-terminal and central domains, respectively [PMID:22277651]. It is a scaffold for both histone deacetylases (HDACs) and C-terminal binding protein (CtBP) corepressors and functions by both HDAC-dependent and -independent mechanisms in a receptor-specific manner[PMID:19744932].

Protocols

Product Specific Protocols
WB protocol for LCOR antibody 14476-1-APDownload protocol
IHC protocol for LCOR antibody 14476-1-APDownload protocol
IF protocol for LCOR antibody 14476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols