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  • KD/KO Validated

LEPR Polyclonal antibody

LEPR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20966-1-AP

Synonyms

CD295, DB, HuB219, LEP R, LEPR, leptin receptor, OB R, OB receptor, OBR



Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, mouse heart tissue, K-562 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse liver tissue, human heart tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20966-1-AP targets LEPR in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name leptin receptor
Calculated Molecular Weight 132 kDa
Observed Molecular Weight 100-150 kDa
GenBank Accession NumberNM_002303
Gene Symbol LEPR
Gene ID (NCBI) 3953
RRIDAB_2878775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hormone leptin, secreted predominantly from adipose tissue, plays a crucial role in the regulation of numerous neuroendocrine functions, from energy homeostasis to reproduction (PMID: 22249808). Leptin acts through the leptin receptor (LEPR, also known as OB-R), a single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, LEPR mediates signaling through JAK2/STAT3 (PMID: 9102398). LEPR plays an important role in the regulation of glucose homeostasis via feeding and adiposity. Alternatively spliced transcript variants encoding different isoforms of LEPR have been described. This antibody can recognize long (LEPRb) and short (LEPRa,c,d) isoforms. The band at 150 kDa detected by this antibody is larger than the predicted molecular weight, probably due to glycosylation (PMID: 10026237).

Protocols

Product Specific Protocols
WB protocol for LEPR antibody 20966-1-APDownload protocol
IHC protocol for LEPR antibody 20966-1-APDownload protocol
IF protocol for LEPR antibody 20966-1-APDownload protocol
IP protocol for LEPR antibody 20966-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cancer Lett

Pharmacological inhibition of KDM1A/LSD1 enhances estrogen receptor beta-mediated tumor suppression in ovarian cancer

Authors - Prabhakar Pitta Venkata
humanWB, IHC

Oncotarget

Leptin signaling enhances cell invasion and promotes the metastasis of human pancreatic cancer via increasing MMP-13 production.

Authors - Yingchao Fan
humanIHC

Cancers (Basel)

Knockdown of Leptin Receptor Affects Macrophage Phenotype in the Tumor Microenvironment Inhibiting Breast Cancer Growth and Progression.

Authors - Luca Gelsomino
humanWB

Comput Struct Biotechnol J

Celastrol inhibits the proliferation and migration of MCF-7 cells through the leptin-triggered PI3K/AKT pathway.

Authors - Pingping Chen
mouseWB

FASEB J

Immunotherapy against angiotensin II receptor ameliorated insulin resistance in a leptin receptor-dependent manner.

Authors - Jiayu Zheng
  • KD Validated
humanIHC

Biomedicines

Leptin, Leptin Receptor, KHDRBS1 (KH RNA Binding Domain Containing, Signal Transduction Associated 1), and Adiponectin in Bone Metastasis from Breast Carcinoma: An Immunohistochemical Study.

Authors - Paola Maroni