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  • KD/KO Validated

LEPR Polyclonal antibody

LEPR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 20966-1-AP

Synonyms

CD295, DB, HuB219, LEP R, LEPR, leptin receptor, OB R, OB receptor, OBR



Tested Applications

Positive WB detected inmouse heart tissue, PC-3 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman prostate hyperplasia tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20966-1-AP targets LEPR in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name leptin receptor
Calculated molecular weight 132 kDa
Observed molecular weight 150 kDa
GenBank accession numberNM_002303
Gene symbol LEPR
Gene ID (NCBI) 3953
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The hormone leptin, secreted predominantly from adipose tissue, plays a crucial role in the regulation of numerous neuroendocrine functions, from energy homeostasis to reproduction (PMID: 22249808). Leptin acts through the leptin receptor (LEPR, also known as OB-R), a single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, LEPR mediates signaling through JAK2/STAT3 (PMID: 9102398). LEPR plays an important role in the regulation of glucose homeostasis via feeding and adiposity. Alternatively spliced transcript variants encoding different isoforms of LEPR have been described. This antibody can recognize long (LEPRb) and short (LEPRa,c,d) isoforms. This antibody detects endogenous LEPR with an apparent molecular weight of 150 kDa, which is larger than the predicted molecular weight, probably due to glycosylation (PMID: 10026237).

Protocols

Product Specific Protocols
WB protocol for LEPR antibody 20966-1-APDownload protocol
IHC protocol for LEPR antibody 20966-1-APDownload protocol
IF protocol for LEPR antibody 20966-1-APDownload protocol
IP protocol for LEPR antibody 20966-1-APDownload protocol
FC protocol for LEPR antibody 20966-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Pineal Res

Leptin mediates the effects of melatonin on female reproduction in mammals.

Authors - Dongying Lv
humanIHC

Materials (Basel)

Advantage of Alveolar Ridge Augmentation with Bioactive/Bioresorbable Screws Made of Composites of Unsintered Hydroxyapatite and Poly-L-lactide.

Authors - Shintaro Sukegawa
ratWB

Mar Drugs

Anti-Obesity Effect of Chitosan Oligosaccharide Capsules (COSCs) in Obese Rats by Ameliorating Leptin Resistance and Adipogenesis.

Authors - Haitao Pan
ratWB,IHC

Acta Pharmacol Sin

Acid sphingomyelinase downregulation alleviates vascular endothelial leptin resistance in rats.

Authors - Bei-Bo Cai
  • KD Validated
mouseWB,IF

Cancer Cell Int

Leptin Receptor (LEPR) promotes proliferation, migration, and invasion and inhibits apoptosis in hepatocellular carcinoma by regulating ANXA7.

Authors - He Huang
mouseWB

J Nutr Biochem

Ablation of β,β-carotene-9',10'-oxygenase 2 remodels the hypothalamic metabolome leading to metabolic disorders in mice.

Authors - Xin Guo