LGALS3BP Antibody 60066-1-Ig

Tested Applications

Applications:

ELISA, WB, IP, IHC, IF
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human blood tissue were subjected to SDS PAGE followed by western blot with 60066-1-Ig( LGALS3BP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IP Result of anti-LGALS3BP (IP:60066-1-Ig, 5ug; Detection:60066-1-Ig 1:1000) with human plasma tissue lysate 4000ug.

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig( LGALS3BP Antibody) at dilution of 1:500 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig( LGALS3BP Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed A431 cells using 60066-1-Ig(LGALS3BP antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:

human

Positive WB detected in:

human blood tissue

Positive IP detected in:

human plasma tissue

Positive IHC detected in:

human breast cancer tissue, human breast cancer tissue, human oesophagus cancer tissue, human skin cancer tissue

Positive IF detected in:

A431 cells

Recommended dilution:

WB : 1:1000-1:4000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:50-1:500

IF : 1:50-1:500

Product Information

Source:

Mouse

Purification method:

Caprylic acid/ammonium sulfate precipitation

Isotype:

IgG2b

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

LGALS3BP fusion protein ag0294

Full name:

lectin, galactoside-binding, soluble, 3 binding protein

Calculated molecular weight:

585aa,65 kDa

Observed molecular weight:

90 kDa

GenBank accession number:

BC002403

Gene ID (NCBI):

3959

Gene symbol

LGALS3BP

Synonyms

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP is a widely expressed, secreted galectin. It has been found elevated in the serum of patients with various types of breast, lung, colorectal, ovary, and endometrial cancer, and in those infected by the human immunodeficiency virus (HIV). High expression levels of LGALS3BP are associated with a shorter survival, the occurrence of metastasis or a reduced response to chemotherapy. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Immunoblotting showed that LGALS3BP is likely contained in breast milk, serum, semen, saliva, urine, and tears.