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LGALS3BP Antibody 0 Publications

Mouse Monoclonal| Catalog number: 60066-1-Ig |CloneNo.: 2D8E11

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Con: 210 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

LGALS3BP Antibody WB human blood tissue 60066-1-Ig

human blood tissue were subjected to SDS PAGE followed by western blot with 60066-1-Ig( LGALS3BP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

LGALS3BP Antibody IP human plasma tissue 60066-1-Ig

IP Result of anti-LGALS3BP (IP:60066-1-Ig, 5ug; Detection:60066-1-Ig 1:1000) with human plasma tissue lysate 4000ug.

LGALS3BP Antibody IHC human breast cancer tissue 60066-1-Ig

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig( LGALS3BP Antibody) at dilution of 1:500 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

LGALS3BP Antibody IHC human breast cancer tissue 60066-1-Ig

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig( LGALS3BP Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

LGALS3BP Antibody IF A431 cells 60066-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed A431 cells using 60066-1-Ig(LGALS3BP antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human
Positive WB detected in:
human blood tissue
Positive IP detected in:
human plasma tissue
Positive IHC detected in:
human breast cancer tissue, human oesophagus cancer tissue, human skin cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
A431 cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Caprylic acid/ammonium sulfate precipitation
Isotype:
IgG2b
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
LGALS3BP fusion protein Ag0294
Full name:
lectin, galactoside-binding, soluble, 3 binding protein
Calculated molecular weight:
585aa,65 kDa
Observed molecular weight:
90 kDa
GenBank accession number:
BC002403
Gene ID (NCBI):
3959
Gene symbol
LGALS3BP
Synonyms
90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP (also known as 90K or Mac-2 BP) is a widely expressed, secreted galectin. It has been found elevated in the serum of patients with various types of breast, lung, colorectal, ovary, and endometrial cancer, and in those infected by the human immunodeficiency virus (HIV). High expression levels of LGALS3BP are associated with a shorter survival, the occurrence of metastasis or a reduced response to chemotherapy. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. LGALS3BP has a predicted mass of ∼65 kDa. It is heavily glycosylated and shows an apparent molecular weight of 90 kDa on SDS-PAGE.


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