CoraLite®594-conjugated LGALS3BP Monoclonal antibody

LGALS3BP Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D8E11

Cat no : CL594-60066

Synonyms

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K



Tested Applications

Positive IF detected inhuman breast cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 60066-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-60066 targets LGALS3BP in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen LGALS3BP fusion protein Ag0294
Full Name lectin, galactoside-binding, soluble, 3 binding protein
Calculated molecular weight 585 aa, 65 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC002403
Gene symbol LGALS3BP
Gene ID (NCBI) 3959
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm / 614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ). LGALS3BP has a predicted mass of ∼65 kDa. It is heavily glycosylated and shows an apparent molecular weight of 90 kDa on SDS-PAGE.

Protocols

Product Specific Protocols
IF protocol for CL594 LGALS3BP antibody CL594-60066Download protocol
Standard Protocols
Click here to view our Standard Protocols