Validation Data Gallery
|Positive WB detected in||mouse heart tissue, rat lung tissue, Jurkat cells, HeLa cells, human heart tissue, rat heart tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15440-1-AP targets LONP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LONP1 fusion protein Ag7196|
|Full Name||lon peptidase 1, mitochondrial|
|Calculated molecular weight||106 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC000235|
|Gene ID (NCBI)||9361|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.
|Product Specific Protocols|
|WB protocol for LONP1 antibody 15440-1-AP||Download protocol|
|IHC protocol for LONP1 antibody 15440-1-AP||Download protocol|
|IF protocol for LONP1 antibody 15440-1-AP||Download protocol|
|IP protocol for LONP1 antibody 15440-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochim Biophys Acta Mol Basis Dis
Mutant huntingtin inhibits the mitochondrial unfolded protein response by impairing ABCB10 mRNA stability.
Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity.
TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.
CDDO-Me Selectively Attenuates CA1 Neuronal Death Induced by Status Epilepticus via Facilitating Mitochondrial Fission Independent of LONP1.
TRPC6-Mediated ERK1/2 Activation Increases Dentate Granule Cell Resistance to Status Epilepticus Via Regulating Lon Protease-1 Expression and Mitochondrial Dynamics.
Fidelity of translation initiation is required for coordinated respiratory complex assembly.