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  • KD/KO Validated

LRPPRC Polyclonal antibody

LRPPRC Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21175-1-AP

Synonyms

130 kDa leucine rich protein, CLONE 23970, GP130, LRP 130, LRP130, LRPPRC, LSFC



Tested Applications

Positive WB detected inHCT 116 cells, mouse heart tissue, HepG2 cells, mouse skeletal muscle tissue, mouse kidney tissue, mouse liver tissue, HeLa cells, rat kidney tissue, rat liver tissue, rat heart tissue, PC-12 cells, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, HepG2 cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21175-1-AP targets LRPPRC in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRPPRC fusion protein Ag15452
Full Name leucine-rich PPR-motif containing
Calculated Molecular Weight 1394 aa, 158 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC050311
Gene Symbol LRPPRC
Gene ID (NCBI) 10128
RRIDAB_10733879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

Protocols

Product Specific Protocols
WB protocol for LRPPRC antibody 21175-1-APDownload protocol
IHC protocol for LRPPRC antibody 21175-1-APDownload protocol
IF protocol for LRPPRC antibody 21175-1-APDownload protocol
IP protocol for LRPPRC antibody 21175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIP

Nat Biotechnol

Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames.

Authors - Xue-Hai Liang
mouseIF,WB,CoIP

Nat Commun

Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA

Authors - Ayesha Sen
humanWB,IF

Nat Commun

A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.

Authors - Peng Sun
humanWB

Genome Biol

Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes

Authors - Iliana Soto
  • KO Validated
humanIHC,CoIP

Redox Biol

LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.

Authors - Wen-Su Wei
humanWB

EMBO J

Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells.

Authors - Yanni Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Angela (Verified Customer) (10-09-2018)

Worked well with 20 ug whole cell lysate run on 7% Tris-Acetate SDS-PAGE. Clean band seen between 120-150 kDa.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:6000
  • Cell Tissue Type: HEK293 cells