Tested Applications
| Positive WB detected in | THP-1 cells, mouse lung tissue, human spleen tissue, HL-60 cells |
| Positive IHC detected in | mouse heart tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 3 publications below |
| IF | See 7 publications below |
Product Information
15013-1-AP targets Lysozyme in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6917 Product name: Recombinant human Lysozyme protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-148 aa of BC004147 Sequence: CELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV Predict reactive species |
| Full Name | lysozyme (renal amyloidosis) |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 15-17 kDa |
| GenBank Accession Number | BC004147 |
| Gene Symbol | Lysozyme |
| Gene ID (NCBI) | 4069 |
| RRID | AB_10639507 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61626 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Lysozyme antibody 15013-1-AP | Download protocol |
| WB protocol for Lysozyme antibody 15013-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Chem Biol Engineered Glycocalyx Regulates Stem Cell Proliferation in Murine Crypt Organoids. | ||
Int J Mol Sci Photo-Crosslinking Hydrogel Based on Porcine Small Intestinal Submucosa Decellularized Matrix/Fish Collagen/GelMA for Culturing Small Intestinal Organoids and Repairing Intestinal Defects | ||
Arch Toxicol Extending the viability of human precision-cut intestinal slice model for drug metabolism studies. | ||
FASEB J Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate. | ||
Arch Med Sci Lysozyme promotes renal fibrosis through the JAK/STAT3 signal pathway in diabetic nephropathy | ||
Int J Mol Sci Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Balawant (Verified Customer) (12-20-2022) | I have used this antibody for colon tissue and colon cell line. this antibody is working great and no background.
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FH Claudio (Verified Customer) (12-15-2021) | The antibody staining did not yield any labelling, though it was tested in paraffin sections so you might get better results with cryostat sections.
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