MBP tag Monoclonal antibody
MBP tag Monoclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
recombinant protein
Applications
WB, IP, IF, ELISA
Conjugate
Unconjugated
CloneNo.
4C6H4
Cat no : 66003-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Recombinant protein |
Positive IP detected in | Recombinant protein protein |
Positive IF detected in | Transfected cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
Product Information
66003-1-Ig targets MBP tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.
Tested Reactivity | recombinant protein |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | MBP tag fusion protein Ag0942 |
Full Name | MBP tag |
Calculated molecular weight | 40 kDa |
Observed molecular weight | 40 kDa |
Gene symbol | |
Gene ID (NCBI) | |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.
Protocols
Product Specific Protocols | |
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WB protocol for MBP tag antibody 66003-1-Ig | Download protocol |
IP protocol for MBP tag antibody 66003-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Virol The extended C-terminal region of influenza C nucleoprotein is important for nuclear import and RNP activity. | ||
Mar Drugs A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate. | ||
J Virol MMP-9 Facilitates Hepatitis B Virus Replication through Binding with IFNAR1 to Repress IFN/JAK/STAT Signaling. | ||
Front Plant Sci Characterization of Ferredoxin-Dependent Biliverdin Reductase PCYA1 Reveals the Dual Function in Retrograde Bilin Biosynthesis and Interaction With Light-Dependent Protochlorophyllide Oxidoreductase LPOR in Chlamydomonas reinhardtii. | ||
PLoS Genet SCFAtPP2-B11 modulates ABA signaling by facilitating SnRK2.3 degradation in Arabidopsis thaliana. | ||
Nat Commun Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet. |