MBP tag Monoclonal antibody, PBS Only

MBP tag Monoclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4C6H4

Cat no : 66003-1-PBS

Synonyms

Maltose Binding Protein, MBP, MBP Tag

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Validation Data Gallery

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  WB analysis of Recombinant protein using 66003-1-Ig (same clone as 66003-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66003-1-Ig (MBP tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66003-1-PBS in a different storage buffer formulation.

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  IP experiment of Recombinant protein using 66003-1-Ig (same clone as 66003-1-PBS)

  IP result of anti-MBP tag (IP:66003-1-Ig, 5ug; Detection:66003-1-Ig 1:20000) with Recombinant protein protein lysate 800ug. This data was developed using the same antibody clone with 66003-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected cells using 66003-1-Ig (same clone as 66003-1-PBS)

  IF result of MBP tag antibody (66003-1-Ig, 1:1,000) with MBP-Tagged protein.Courtesy of Neeraj Tiwari, PhD, Yale School of Medicine, Yale University. This data was developed using the same antibody clone with 66003-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66003-1-PBS targets MBP tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MBP tag fusion protein Ag0942
• Full Name: MBP tag
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.