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KD/KO Validated

ME2 Polyclonal antibody

Name: ME2 Polyclonal antibody
Brand: Proteintech
SKU: 24944-1-AP

ME2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 24944-1-AP

Synonyms

Malic enzyme 2, ME2, NAD ME

Validation Data Gallery

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AllWBIPIHC

WB analysis using 24944-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 24944-1-AP
WB result of ME2 antibody (24944-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ME2 transfected HepG2 cells.
WB analysis using 24944-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 24944-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 24944-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 24944-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 24944-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 24944-1-AP
IP result of anti-ME2 (IP:24944-1-AP, 4ug; Detection:24944-1-AP 1:500) with HeLa cells lysate 3200ug.
IHC staining of human pancreas cancer using 24944-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24944-1-AP (ME2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 24944-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24944-1-AP (ME2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 24944-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24944-1-AP (ME2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon using 24944-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24944-1-AP (ME2 Antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 24944-1-AP

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Tested Applications

Positive WB detected in	HL-60 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human pancreas cancer tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IP	See 1 publications below

Product Information

24944-1-AP targets ME2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ME2 fusion protein Ag20741
Full Name	malic enzyme 2, NAD(+)-dependent, mitochondrial
Calculated Molecular Weight	65 kDa
Observed Molecular Weight	54 kDa, 65 kDa
GenBank Accession Number	BC000147
Gene Symbol	ME2
Gene ID (NCBI)	4200
RRID	AB_2879813
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.

Protocols

Product Specific Protocols
WB protocol for ME2 antibody 24944-1-AP	Download protocol
IHC protocol for ME2 antibody 24944-1-AP	Download protocol
IP protocol for ME2 antibody 24944-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Authors - Linchong Sun View Article
human	WB	Adv Sci (Weinh) SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma Authors - Qifan Hu View Article
human	WB	Cell Death Differ SIRT5-mediated ME2 desuccinylation promotes cancer growth by enhancing mitochondrial respiration Authors - Peng Teng KD Validated View Article
human	WB,IP	Nat Commun AKT1 phosphorylation of cytoplasmic ME2 induces a metabolic switch to glycolysis for tumorigenesis Authors - Taiqi Chen View Article

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ME2 Polyclonal antibody

ME2 Polyclonal Antibody for IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 24944-1-AP

WB analysis of HepG2 using 24944-1-AP

WB analysis using 24944-1-AP

WB analysis of Jurkat using 24944-1-AP

WB analysis of Jurkat using 24944-1-AP

WB analysis of HL-60 using 24944-1-AP

WB analysis of HeLa using 24944-1-AP

IP experiment of HeLa using 24944-1-AP

IHC staining of human pancreas cancer using 24944-1-AP

IHC staining of human pancreas cancer using 24944-1-AP

IHC staining of human colon using 24944-1-AP

IHC staining of human colon using 24944-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma

SIRT5-mediated ME2 desuccinylation promotes cancer growth by enhancing mitochondrial respiration

AKT1 phosphorylation of cytoplasmic ME2 induces a metabolic switch to glycolysis for tumorigenesis