MMP13 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 18165-1-AP
CLG3, Collagenase 3, Matrix metalloproteinase 13, MMP 13, MMP13
|Positive WB detected in||HeLa cells, MCF-7 cells, MDA-MB-453s cells, zebrafish tissue, mouse liver tissue, rat liver tissue|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
18165-1-AP targets MMP13 in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Reactivity||human, mouse, rat|
|Cited Species||human, mouse, rabbit, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MMP13 fusion protein Ag12653|
|Full Name||matrix metallopeptidase 13 (collagenase 3)|
|Calculated molecular weight||471aa,54 kDa|
|Observed molecular weight||65-70 kDa, 50-55 kDa, 40 kDa|
|GenBank accession number||BC074808|
|Gene ID (NCBI)||4322|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
MMP-13(Matrix metalloproteinase-13), also known as collagenase-3, cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial collagenase (MMP-1). MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).
|Product Specific Protocols|
|WB protocol for MMP13 antibody 18165-1-AP||Download protocol|
|IHC protocol for MMP13 antibody 18165-1-AP||Download protocol|
|IF protocol for MMP13 antibody 18165-1-AP||Download protocol|
|IP protocol for MMP13 antibody 18165-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Effects of IL-1β on MMP-9 Expression in Cementoblast-Derived Cell Line and MMP-Mediated Degradation of Type I Collagen.
Int J Oncol
25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway.
Cortistatin binds to TNF-α receptors and protects against osteoarthritis.
J Cell Physiol
Reduced PDGF-AA in subchondral bone leads to articular cartilage degeneration after strenuous running.
miR-93-5p attenuates IL-1β-induced chondrocyte apoptosis and cartilage degradation in osteoarthritis partially by targeting TCF4.
miR-100-5p-abundant exosomes derived from infrapatellar fat pad MSCs protect articular cartilage and ameliorate gait abnormalities via inhibition of mTOR in osteoarthritis.