MMP13 Antibody

MMP13 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

63

Cat no : 18165-1-AP

Synonyms

CLG3, Collagenase 3, Matrix metalloproteinase 13, MMP 13, MMP13



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, MDA-MB-453s cells, zebrafish tissue, mouse liver tissue, rat liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

18165-1-AP targets MMP13 in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse, rabbit, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP13 fusion protein Ag12653
Full Name matrix metallopeptidase 13 (collagenase 3)
Calculated molecular weight 471aa,54 kDa
Observed molecular weight 65-70 kDa, 50-55 kDa, 40 kDa
GenBank accession number BC074808
Gene symbol MMP13
Gene ID (NCBI) 4322
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP-13(Matrix metalloproteinase-13), also known as collagenase-3, cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial collagenase (MMP-1). MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).

Protocols

Product Specific Protocols
WB protocol for MMP13 antibody 18165-1-APDownload protocol
IHC protocol for MMP13 antibody 18165-1-APDownload protocol
IF protocol for MMP13 antibody 18165-1-APDownload protocol
IP protocol for MMP13 antibody 18165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Inflammation

Effects of IL-1β on MMP-9 Expression in Cementoblast-Derived Cell Line and MMP-Mediated Degradation of Type I Collagen.

Authors - Mingyuan Du
humanWB

Int J Oncol

25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway.

Authors - Saisai Wang
humanWB

EBioMedicine

Cortistatin binds to TNF-α receptors and protects against osteoarthritis.

Authors - Yunpeng Zhao
mouseIHC

J Cell Physiol

Reduced PDGF-AA in subchondral bone leads to articular cartilage degeneration after strenuous running.

Authors - Zilong Yao
humanWB

Bone

miR-93-5p attenuates IL-1β-induced chondrocyte apoptosis and cartilage degradation in osteoarthritis partially by targeting TCF4.

Authors - Huaming Xue
mouseIHC

Biomaterials

miR-100-5p-abundant exosomes derived from infrapatellar fat pad MSCs protect articular cartilage and ameliorate gait abnormalities via inhibition of mTOR in osteoarthritis.

Authors - Jiangyi Wu