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  • Featured Product
  • KD/KO Validated

MPI Polyclonal antibody

MPI Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14234-1-AP

Synonyms

Phosphomannose isomerase, Phosphohexomutase, Mannose-6-phosphate isomerase, mannose phosphate isomerase, Mannose 6 phosphate isomerase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MPI Polyclonal antibody (14234-1-AP)

    WB analysis using 14234-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14234-1-AP (MPI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MPI Polyclonal antibody (14234-1-AP)

    WB analysis of human brain using 14234-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14234-1-AP (MPI antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using MPI Polyclonal antibody (14234-1-AP)

    IP experiment of mouse brain using 14234-1-AP

    IP result of anti-MPI (IP:14234-1-AP, 4ug; Detection:14234-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using MPI Polyclonal antibody (14234-1-AP)

    IF Staining of NIH/3T3 using 14234-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 14234-1-AP (MPI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using MPI Polyclonal antibody (14234-1-AP)

    IF Staining of NIH/3T3 using 14234-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MPI antibody (14234-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14234-1-AP targets MPI in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MPI fusion protein Ag5475
Full Name mannose phosphate isomerase
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 40-47 kDa
GenBank Accession NumberBC046357
Gene Symbol MPI
Gene ID (NCBI) 4351
RRIDAB_2146302
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MPI(mannose-6-phosphate isomerase) is also named as PMI1 and belongs to the mannose-6-phosphate isomerase type 1 family. It is a zinc metalloenzyme that catalyzes the reversible isomerization of mannose-6-phosphate and fructose-6-phosphate. MPI plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. This proterin has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MPI antibody 14234-1-APDownload protocol
IF protocol for MPI antibody 14234-1-APDownload protocol
IP protocol for MPI antibody 14234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Mannose impairs tumour growth and enhances chemotherapy.

Authors - Pablo Sierra Gonzalez
  • KD Validated
View Article
ratWB

Mil Med Res

D-mannose alleviates intervertebral disc degeneration through glutamine metabolism

Authors - Zheng-Lin Dong
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000