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NCOA5 Polyclonal antibody

NCOA5 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, FC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 20175-1-AP

Synonyms

CIA, KIAA1637, NCoA 5, NCOA5, nuclear receptor coactivator 5



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IF detected inHela cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20175-1-AP targets NCOA5 in WB, IP, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCOA5 fusion protein Ag14014
Full Name nuclear receptor coactivator 5
Calculated Molecular Weight 579 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC056872
Gene Symbol NCOA5
Gene ID (NCBI) 57727
RRIDAB_10694674
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptor coactivator 5 (NCOA5), also known as coactivator independent of AF-2 (CIA), possesses both repressor and activator functions (PMID: 11113208). It can interact with Estrogen Receptor (ER) alpha and ER beta in a ligand-dependent manner. NCOA5 has been shown to regulate the ER alpha-mediated transcription as well as the expression of the c-Myc gene (PMID: 11113208; 15073177). Recently identified as a conserved component of pluripotent stem cells, mammalian NCOA5 may contribute to the maintenance of the pluripotent stem cell population (PMID: 24268775).

Protocols

Product Specific Protocols
WB protocol for NCOA5 antibody 20175-1-APDownload protocol
IF protocol for NCOA5 antibody 20175-1-APDownload protocol
IP protocol for NCOA5 antibody 20175-1-APDownload protocol
FC protocol for NCOA5 antibody 20175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Rep

SILAC Proteomics of Planarians Identifies Ncoa5 as a Conserved Component of Pluripotent Stem Cells.

Authors - B?ser Alexander A
bovineWB,IF,ChIP

Biochem Biophys Res Commun

NCOA5 is a master regulator of amino acid-induced mTOR activation and β-casein synthesis in bovine mammary epithelial cells.

Authors - Xiaohan Yuan
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