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NME7 Polyclonal antibody

NME7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26449-1-AP

Synonyms

NDK 7, NDP kinase 7, NME7

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse embryo tissue using NME7 Polyclonal antibody (26449-1-AP)

    WB analysis of mouse embryo using 26449-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 26449-1-AP (NME7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using NME7 Polyclonal antibody (26449-1-AP)

    IHC staining of human brain using 26449-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26449-1-AP (NME7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using NME7 Polyclonal antibody (26449-1-AP)

    IHC staining of human brain using 26449-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26449-1-AP (NME7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse embryo tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26449-1-AP targets NME7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NME7 fusion protein Ag24918
Full Name non-metastatic cells 7, protein expressed in (nucleoside-diphosphate kinase)
Calculated Molecular Weight 376 aa, 42 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC006983
Gene Symbol NME7
Gene ID (NCBI) 29922
RRIDAB_2880517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NME7, also named as NDK7, nm23-H7, plays the major role in the synthesis of nucleoside triphosphates other than ATP. NME7 has two isoforms with MW 38-42 kDa.

Protocols

Product Specific Protocols
WB protocol for NME7 antibody 26449-1-APDownload protocol
IHC protocol for NME7 antibody 26449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols