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NPL Polyclonal antibody

NPL Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16715-1-AP

Synonyms

c112, C1orf13, N acetylneuraminate lyase, NALase, NPL, Sialate lyase, Sialate pyruvate lyase, Sialic acid aldolase, Sialic acid lyase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NPL Polyclonal antibody (16715-1-AP)

    WB analysis using 16715-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16715-1-AP (NPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NPL Polyclonal antibody (16715-1-AP)

    WB analysis using 16715-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16715-1-AP (NPL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using NPL Polyclonal antibody (16715-1-AP)

    WB analysis of Raji using 16715-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 16715-1-AP (NPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NPL Polyclonal antibody (16715-1-AP)

    IF Staining of MCF-7 using 16715-1-AP

    Immunofluorescent analysis of MCF-7 cells, using NPL antibody 16715-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse brain tissue, mouse kidney tissue, Raji cells, rat brain tissue, rat kidney tissue
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16715-1-AP targets NPL in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPL fusion protein Ag10137
Full Name N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase)
Calculated Molecular Weight 27 kDa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC058003
Gene Symbol NPL
Gene ID (NCBI) 80896
RRIDAB_2154866
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The N-acetylneuraminate lyase(NPL) from human (hNAL) which consists of 320 amino acids, has a molecular mass of 35.2 kDa and it catalyses the reversible aldol reaction of sialic acid, and has been extensively used in the synthesis of sialic acids and its analogues(PMID:19057931). The enzyme is widely distributed and are present in numerous pro- and eukaryotic cells, in which they are localized only in the cytosol(PMID:12453637). It belongs to the DHDPS family and has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for NPL antibody 16715-1-APDownload protocol
IF protocol for NPL antibody 16715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols