Tested Applications
| Positive WB detected in | mouse brain tissue, mouse kidney tissue, Raji cells, rat brain tissue, rat kidney tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16715-1-AP targets NPL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10137 Product name: Recombinant human NPL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC058003 Sequence: MAFPKKKLQGLVAATITPMTENGEINFSVIGQYVDYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEWVTKGKDKLDQVIIHVGALSLKESQELAQHAAEIGADGIAVIAPFFLKPWTKDILINFLKEVAAAAPALPFYYYHIPALTGVKIRAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQ Predict reactive species |
| Full Name | N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) |
| Calculated Molecular Weight | 27 kDa, 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC058003 |
| Gene Symbol | NPL |
| Gene ID (NCBI) | 80896 |
| RRID | AB_2154866 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXD5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The N-acetylneuraminate lyase(NPL) from human (hNAL) which consists of 320 amino acids, has a molecular mass of 35.2 kDa and it catalyses the reversible aldol reaction of sialic acid, and has been extensively used in the synthesis of sialic acids and its analogues(PMID:19057931). The enzyme is widely distributed and are present in numerous pro- and eukaryotic cells, in which they are localized only in the cytosol(PMID:12453637). It belongs to the DHDPS family and has 5 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NPL antibody 16715-1-AP | Download protocol |
| IHC protocol for NPL antibody 16715-1-AP | Download protocol |
| WB protocol for NPL antibody 16715-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NPL Polyclonal antibody (Cat# 16715-1-AP) has 1 citation. It was cited in Cell Stem Cell (IF 23.3) in a study on MMRN1-EGFR-driven sialylglycan-Siglec immune evasion in AML leukemia stem cells. The research field encompasses acute myeloid leukemia (AML), immune evasion mechanisms, and stem cell biology. The antibody was used for Western blot in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell MMRN1-EGFR drives sialylglycan-Siglec immune evasion in AML leukemia stem cells. |
